摘要
作者检测99例手术切除的肝细胞癌(HCC)中R53蛋白和PCNA的表达。99例中合并肉眼下门静脉癌栓(TTPV)39例,合并镜下TTPV30例,无TTPV30例。总体R53表达阳性率为24.24%,PCNA标记指数(PCNALI)为34.6±23.6。在上述三组中,P53表达阳性率、PCNALI差异均有显著性,PCNALI与R53表达密切相关,P53表达阳性的HCC,其PCNALI显著高于P53表达阴性者。结果提示,P53基因突变,PCNA活性增强,HCC增殖水平由此而升高,可能是HCC细胞获得更强侵袭性的重要机制之一,因而也是TTPV形成的机制之一。
Ninety-nine surgical specimens of hepatocellular carcinoma(HCC)were used to determine the expression of P53 protein and that of proliferating,cell nuclear antigen (PCNA).Among them,39 were accompanied by macroscopic tumor thrombi in the portal vein(TTPV),30 with microscopic TTPV and 30 without.The positive rate of P53 expression in 99 HCC specimens was 24.24%, and the mean PCNA Iabel index (PCNA LI) was 34.6±23.6 .The difference of positive rates of P53 expression as well as the PCNA LI in three groups of HCC is significant statistically. There was a strong correlation between P53 expression and PCNA LI. PCNA LI was higher in group with positive expression of P53 than that with negative expression. The results implicate that P53 mutation,which may induce increase of PCNA activity and result in higher proliferative and invasive potentials, is one of the mechanisms of the genesis of TTPV.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1996年第2期75-78,共4页
Chinese Journal of Digestion