摘要
本文报告用ＲＰ－ＨＰＬＣ法测定妇霉栓剂中的有效成分没食子酸的含量。在２７３ｎｍ波长处用紫外检测器检测，没食子酸在１０～８０μｇ／ｍｌ范围内线性关系良好，ｒ＝０．９９９７９，日内、日间精密度（Ｘ±ＲＳＤ）分别为（１００．３±０．９）％、（１０１．９±１．３）％。并对乙醇浸泡提取时间及方法稳定性进行了考察。经对１０批样品的测定表明，栓剂中没食子酸的最高含量为１．０８０ｍｇ／ｇ，最低含量０．８０５１ｍｇ／ｇ。测定方法简单、重现性好。
The gallic acid content in Fu Mei Suppository was determined by RP-HPLC. The detection wavelength was 273nm. The linear relationship between concentration and peak height wasobtained in the concentration range of 10～80μg/ml. The average recoveries within-day and between-days were (100. 3±0. 9)% and (101. 9±1. 3)%, respectively. The time after the Fu Mei Suppository was dissolved in absolute ethanol and the reliability of the method were observed. Among 10 samples the lowest content was 0. 8051mg/g, the highest was 1. 080mg/g.The results showed that the method was simple, rapid and of excellent precision.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1996年第2期105-108,共4页
Chinese Journal of Pharmaceutical Analysis
