摘要
目的:探讨人乳头状瘤病毒(HPV)负荷量与子宫颈癌及其癌前病变的关系.方法:2003年3月~2003年12月在我院行宫颈癌筛查的2 651例患者为研究对象,采用杂交捕获2代(HCⅡ)方法定量检测宫颈HPV DNA的含量(以此表示宫颈HPV负荷量).按HPV DNA含量分成5组,分别为:HPV<1,1≤HPV≤100,101≤HPV≤500,501≤HPV≤1 000,HPV>1 000.对其中HPV DNA阳性妇女,随后进行阴道镜检查、宫颈多点活检和颈管诊刮术.结果:794例阳性患者中的783例进行了阴道镜下多点活检和细胞学诊断,其中子宫颈炎650例(83.23%),CIN 102例(13.47%),宫颈癌26例(3.3%).650例对照组、102例癌前病变组和26例宫颈癌组中,3组的HPV DNA定量总体分布有显著差别(P<0.005).在HPV-DNA定量>100时,宫颈癌前病变的阳性率显著升高(P<0.005);当HPV-DNA的定量>500时,宫颈癌的阳性率显著升高(P<0.001).CINⅡ、CINⅢ及宫颈癌在HPV DNA定量>1时的诊断阳性率较显著<1时显著高(P<0.01).结论:HCⅡ方法是检测宫颈HPV感染的有效手段,HPV DNA含量随宫颈病变的严重程度而增加,可作为宫颈癌前病变及宫颈癌的筛查及病情追踪监测的1项客观指标.
Objective: To explore the relationship between loads of human papilloma virus in cervical carcinoma and cervical intraepithelial neoplasia. Methods: From March 2001 to October 2003, 2651 women who were screened high risk types of human papillomavirus (HPV) infection using hybrid capture (HC Ⅱ) . All patients were divided five groups according to the viral load of HPV -DNA , including: HPV 〈 1, 1 ≤ HPV ≤ 100, 101 ≤ HPV ≤500, 501≤ HPV≤1 000, HPV 〉 1 000. Results: Among 794 cases with positive human papilloma virus , 783 cases had been underwent colposcopy and multi - focal directed punch biopsies plus endoeervieal curettage. Of them , 650 (83. 23% ) cases who were normal or cervicitis as control group; 102 ( 13. 47% ) women diagnosed as cervical intraepithelial neoplasia (CIN) , and 26 (3. 3% ) women diagnosed as cervical squamous cell carcinoma (SCC). Compared three groups, the collectivity distribution of HPV quantity were significantly different. When HPV DNA 〉 100, the rate of diagnosing cervical intraepithelial neoplasia was significantly higher than other groups ; and HPV DNA 〉 500, the masculine rate of diagnosing cervical squamous cell carcinoma was significantly higher ( P 〈 0.005 ) . When HPV DNA 〉 1, the the masculine rate of diagnosing CIN Ⅱ, CIN Ⅲ and SCC were significantly higher than HPV DNA 〈 1 ( P 〈 0.01 ) . Conclusion: The new technology using HC Ⅱ is an effective method to detect HPV infection in cervix. Viral loads of HPV - DNA increase with severity of cervical neoplasia. So it could be used for screening primary cervical carcinoma.
出处
《中国妇幼保健》
CAS
北大核心
2006年第11期1557-1559,共3页
Maternal and Child Health Care of China
关键词
人乳头状瘤病毒
病毒载量
宫颈癌
癌前病变
Papillomavirus
Viral load
Cervixneoplasms
Precancerous conditions