摘要
目的寻求应用不同初筛试验的组合替代传统酶联免疫吸附试验(ELISA)+免疫蛋白印迹试验(WB)的人类免疫缺陷病毒(HIV)抗体检测方法。方法将1 004份血清样本用ELISA和快速试验按平行试验和顺序试验分别进行检测,以WB为金标准,对结果进行比较。结果1 004份受检血清中,平行试验的敏感度和阴性预测值均为100.0%,而顺序试验则为99.8%和99.7%,2种试验的特异度和阳性预测值相同,分别为98.8%和99.4%。结论结合流行率的影响,平行试验更适用于人群的个体诊断、自愿咨询检测(VCT)及要求漏诊率很低的血液筛查(如血站、医院的献血员筛查),但费用较高。而顺序试验的检测成本低,在流行率比较低或资源比较贫困的地区更为适用。
Objective To seek some combinations of different screening reagents to alternate traditional HIV（human immunodeficiency virus） antibody testing methods, a combination of enzyme - linked immunesorbent assays（ELISAs） and western blot（WB）. Methods One thousand and four serum samples were detected by both ELISA and rapid testing according serial and parallel methods which were compared with western blot as gold standard. Results In 1004 serum samples, the sensitivity and negative predictive value of parallel method were same as 100.0 %, while the ones of serial method were 99.8%和99.7%. The specitivity and positive predictive value of the two methods were same as 98.8% 和 99.4%. Concision Parallel method was more suitable to be used for personnel diagnosis, consultation after testing and blood screening if the prevalence rate was considered. Serial method made the testing cost reduced and the testing time shorten, and its negative results were more stronger evidence of the true negative person in low prevalence area.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2006年第6期678-679,共2页
Chinese Journal of Public Health
基金
卫生部艾滋病防治应用性研究项目(WA2003-17)
关键词
人类免疫缺陷病毒(HIV)
抗体检测
平行试验
顺序试验
替代方法
human immunodeficiency virus（HIV）
antibody testing
parallel method
serial method
alternative confirmatory strategies
