摘要
目的建立多引物检测鼠疫耶尔森菌(鼠疫菌)的PCR(M-PCR)方法。方法合成4对引物,分别来源于质粒和染色体DNA上F1、pla、HmsI、nv基因,对164株鼠疫菌进行扩增。结果在164株鼠疫菌中,有152株菌4种基因扩增均阳性,仅云南省12株Hms基因扩增为阴性。结论采用M-PCR方法检测鼠疫菌DNA具有较好的敏感性、特异性和稳定性,其可作为检测与鉴别鼠疫菌和疫情监测方面快速诊断的方法。
Objective To develop a polymerase chain reaction （PCR） method to detect Yersina pestis by multiplex primers （M-PCR）. Methods Four pairs of primers, originated from the genes F1 （specific capsular antigen fraction 1）, pla （palsrninogen activator）, Hrns and lnv encoded on the two kinds of 65×10^6 plasmids and two chromosomal DNA, were designed and 164 strains of Y. pestis were amplified with multiplex primers. Results One hundred and fifty-two of 164 strains of Y. 1）estis showed positive in M-PCR, Only 12 strains of them isolated from Yunnan were negative with amplification of the Hms gene. Conclusion M-PCR method showed satisfactory sensitivity, specificity and stability for detecting and identifying Y. pestis DNA and could be used in surveillance and ranid diagnosis for plague.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2006年第3期221-223,共3页
Chinese Journal of Vector Biology and Control
关键词
鼠疫耶尔森菌
毒力基因
多引物
聚合酶链反应
Yersina pestis
Virulence gene
Multiplex primers
Polymerase chain reaction
