摘要
利用细菌16S rDNA保守序列通用引物对16sF/16sR对苜蓿细菌性萎蔫病菌的标准菌株(IPQ0019)总DNA进行扩增,得到了大约1.5 kb的片段。将此片段插入到pGEM-T载体并转化进大肠杆菌DH5α菌株中,经PCR鉴定、限制性内切酶分析及核苷酸序列同源性分析均表明克隆成功。该研究为制备苜蓿细菌性萎蔫病菌的DNA分子探针奠定了基础。
A 1.5 kb DNA fragment of the standard strain of Clavibacter michiganensis subsp, insidiosus was obtained using universal primers 16sF/16sR based on the conserved region of the 16S rDNA of bacteria. The amplified fragment was inserted into a pGEM-T vector and verified by PCR, restriction endonuclease digestion, and sequence analysis. The study laid the foundation for designing a molecular probe to C. michiganensis subsp, insidiosus.
出处
《草业学报》
CSCD
2006年第3期123-127,共5页
Acta Prataculturae Sinica
基金
国家质检总局植物病原检测专项(Z2000-3-182)资助
