摘要
目的：建立一种同时测定尿中钙、磷、尿酸浓度的方法—血生化分析法,并评价此方法在临床研究中的价值。方法：收集结石患者24h尿液,用浓盐酸酸化和稀释处理后,用生化分析仪测定其中钙、磷浓度,再以氢氧化钠碱化和稀释后测定尿酸浓度。结果：预处理后的尿液中三种物质的测定值均落在生化分析仪的线性范围以内,且均与比色法有显著的相关性。其批内变异系数和日间变异系数均小于5%,回收率均大于95%。结论：此方法简单、准确、快速,适用于临床上尿石症患者结石形成原因的诊断。为临床医师制定个体化的尿石预防方案提供依据。
Objective：To establish a method for simultaneous assay of calcium, phosphonium and uric acid in urine by automatic biochemical analyzer and evaluate its value in clinic research. Methods：Twenty four hours urine was taken from urinary calculus patients and acidified by concentrated hydrochloric acid and diluted, concentrations of calcium and phosphonium were measured by biochemical analyzer. Alkalinized by natrium hydroxydatum and diluted to measure concentration of uric acid. Results： The determination results of calcium, phosphonium and uric acid in treated urine were all in linear ranger of biochemical analyzer. This method had a significant correlation with colorimetric method. All the interassay and between assay coefficients of variation were less than 5 %. The re coveries for the three urine samples were all more than 95%. Conclusions： This method was simple, accurate and rapid. It was applied to diagnose the cause of calculus in urinary calculus patients and provided evidence for doctor to institute individual project for preventing urinary calculus.
出处
《临床泌尿外科杂志》
2006年第6期461-463,共3页
Journal of Clinical Urology
关键词
尿结石
血生化分析
预处理
Urine gravel
Biochemical analyzer
Preconditioning
