摘要
目的:研究白细胞介素(interleukin,IL)17对中性粒细胞凋亡的影响并探讨其可能的内在机制。方法:分离培养健康人外周血中性粒细胞,分别加入IL17、热灭活IL17(XIL17)和地塞米松,并设立对照组。通过形态学观察和DNA片段化的检测分析凋亡情况;利用免疫细胞化学方法检测中性粒细胞Bax蛋白的表达。结果:培养中对照组中性粒细胞呈现出凋亡极具特征性的形态学改变;随时间延长,凋亡指数(apoptosisindex,AI)呈上升趋势,0h为(1.54±0.08)%,12h为(11.48±1.80)%(与0h比较,P<0.05),24h为(34.19±1.92)%(与0h比较,P<0.01)。在50μg/L质量浓度时IL17促进中性粒细胞凋亡,0,12,24h的AI分别为(1.43±0.17)%(与对照组0h比较,P>0.05),(20.47±6.22)%(与对照组12h比较,P<0.01)和(40.74±3.48)%(与对照组24h比较,P<0.05);而IL17在质量浓度为5μg/L和0.5μg/L浓度时可抑制凋亡,24h的AI分别为(14.24±4.26)%和(19.86±4.39)%,与对照组24h比较,P均<0.01。50μg/LXIL17组24h的AI为(33.22±1.61)%(与对照组24h比较,P>0.05)。中性粒细胞凋亡的同时伴有DNA的片段化。免疫化学染色显示同期中性粒细胞Bax蛋白表达量与AI呈强正相关(r=0.932,P<0.01)。结论:体外IL17对中性粒细胞的凋亡有调节作用,在较高浓度下加速凋亡,在较低浓度下延缓凋亡。IL17对Bax蛋白表达的调控可能是其内在机制之一。
Objective: To study the effect of Interleukin(IL)-17 on neutrophil apoptosis and try to explain the possible mechanism involved. Methods: Neutrophils isolated from healthy donors were incubated in enriched RPMI 1640 cell culture medium at 37℃ in 5% carbon dioxide. Subgroups were incubated with IL-17, heat-denatured IL-17 (X-IL-17), dexamethasone (DEX), or buffer alone. Apoptosis was assessed by morphologic changes, by detecting DNA strand breaks. Production of proapoptotic protein Bax by neutrophils was evaluated by immunocytochemistry. Results: At the time of neutrophil incubation, neutrophils in the control subsets exhibited morphologic evidence of apoptosis. A steady rise in apoptosis index (AI) was noted, with ( 1.54±0.08 ) % for 0 h, ( 11.48 ± 1.80 ) % ( compared with 0 h, P 〈 0.05 ) for 12 h and (34.19 ±1.92) % ( compared with 0 h, P 〈 0.01 ) for 24 h, respectively. IL-17 at concentration of 50μg/L resulted in increased AI of neutrophils, with ( 1.43 ± 0.17 ) % ( compared with control 0 h, P 〉 0.05 ) for 0 h, (20.47±6.22 ) % ( compared with control 12 h, P 〈 0.01 ) for 12 h and (40.74± 3.48)% (compared with control 24 h, P 〈0.05 ) for 24 h, respectively. While at concentrations of 5μg/L and 0.5μg/L, IL-17 resulted in decreased AI, with ( 14.24±4. 26 ) % ( compared with control 24 h, P 〈 0.01 ) for 24 h, and ( 19.86±4.39 ) % ( compared with control 24 h, P 〈0.01 ) for 24 h, respectively. AI of neutrophils treated with X-IL-17 at concentration of 50 ng/ml for 24 h was (33.22 ±1.61 ) % ( compared with control 24 h, P 〉 0.05 ). Neutrophils apoptosis was accompanied by DNA fragmentation. In all groups, the increasing of Bax immunoreactivity was strongly related with more apoptotic neutrophils ( r= 0. 932, P 〈 0.01 ). Conclusion: In vitro, IL-17 modulates apoptosis of neutrophils. At higher concentrations, it accelerates neutrophils apoptosis. At lower concentrations, it delays neutrophils apoptosis. Modulation of the expression of Bax by IL-17 may be one of the possibleinner mechanisms.
出处
《北京大学学报(医学版)》
CAS
CSCD
北大核心
2006年第3期305-309,共5页
Journal of Peking University:Health Sciences
基金
国家自然科学基金(30471915)
北京市自然科学基金(7012022)资助~~