摘要
采用丙酮粉沉淀抽滤法从澳洲青苹中提取多酚氧化酶。提取的粗酶液经30%～90%饱和度硫酸铵分级沉淀、透析除盐、交联葡聚糖G-75凝胶色谱层析、后经PEG6000浓缩,得到纯化的多酚氧化酶,纯化倍数为7.49。纯化后的酶液经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳,采用考马斯亮蓝R-250染色显示一条电泳带。通对过PPO与邻苯二酚反应产物的波谱扫描显示,其产物在λ=410nm下有最高吸收。
polyphenol oxidase （PPO） was extracted from Granny Smith with acetone. The crude enzyme extract was fractionated with solid ammonium sulfate of 30 %-90 % saturation. After dialysis,the enzyme solution was purified by sephadex G-75 column chromatography respectively. The enzyme was concentrated by PEG 6000.A7.49 fold purification of PPO was obtained. The result from polyacrylamide gel electrophoresis of purified enzyme indicated that only one band was found using coomassie brilliant blue R250 stain. The largest absorbion of the product of the reaction between PPO and catechol was at λ =410 nm.
出处
《食品研究与开发》
CAS
北大核心
2006年第5期180-182,共3页
Food Research and Development
基金
国家"十五"科技攻关项目(2001BA501A5-2.3)
霍英东基金项目(2002-81065)
关键词
澳洲青苹
多酚氧化酶
纯化
granny smith
polyphenol oxidase
purification
