摘要
为获得产酶量高、产酶快的菌株，采用碘液染色法从土样分离到1株产β-甘露聚糖酶量高的菌株Bacillus sp．N-6，通过16SrDNA序列相似性比较及生理生化性状鉴定为枯草芽抱杆菌；为得到最佳产酶配方进行了发酵条件优化。结果表明：100mL培养基加入2g魔芋粉、1g酵母粉、1g豆饼粉、起始pH8．0、32c150r／min振荡培养18h，产酶量最高，达316．53U／mL。该酶在pH5．0～9．0和40～55℃下稳定，作用最适条件pH6．0和50℃。CO^2＋对酶有激活作用，Fe^3＋，Ag^＋和EDTA对酶有较强抑制作用。
N-6 strain of high extracellular β-mannanase activity was isolated from soil samples by iodine dyed method in order to obtain the strain which rapidly produced mannanase. The strain was identified as Bacillus subtilis by 16S rDNA BLAST in the Genbank, morphological and physiological characteristics analysis, The result showed that the activity was 316..53 U/mL in the 100 mL culture medium, containing 2 g Konjak powder, 1 g yeast extract, 1 g bean cake powder. The optimal pH, temperature, agitation speed and culture time were 8.0, 32 ℃, 1.50 r/min and 18 h, respectively. The activity was stable at pH 5.0-9.0 and below 55℃ . The optimal condition of the enzyme was pH 6.0 and 50℃, respectively, The enzyme activity was enhanced by Co^2＋ and was inhibited by Fe^3＋ , Ag^＋ and EDTA.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2006年第3期36-40,共5页
Journal of China Agricultural University
基金
澳大利亚国际农业中心资助项目(PHT/1998/140)
