摘要
目的探讨四硫化四砷(As4S4)在体外对NB4细胞增殖的抑制作用。方法As4S4(处理)NB4细胞后,应用台盼蓝排斥实验测定细胞活力,用MTT实验测定细胞增殖抑制率,并观察As4S4对NB4细胞集落形成能力的影响。结果NB4细胞在As4S4存在下进行培养,其细胞活力受到显著抑制,应用MTT实验检测到的As4S4对NB4细胞增殖抑制作用呈剂量依赖性,半数抑制浓度为2.86μmol/L。同时As4S4以剂量依赖的方式抑制NB4细胞的集落形成能力。结论As4S4在体外具有降低NB4细胞活力、抑制NB4细胞增殖及其克隆形成能力的作用。
Objective To study the inhibitory effect of tetra - arsenic tetra - sulfide As4S4 on NB4 cell proliferation. Methods After NB4 cells treated with As4S4, cell viability was assessed using Trypan blue exclusion assay. Inhibitory rate of As4S4 on NB4 cell proliferation was observed by MTT assay. The effect of As4S4 on the colony - forming ability was studied by colony formation of NB4 cells. Results Cell viability was signif icantly inhibited when NB4 cells cultured in the presence of As4S4. The inhibitory effect of As4S4 on NB4 cellproliferation was in a manner of dose -dependent, with an IC50 of 2.86μmol/L. Meanwhile, As4S4 inhibited the colony forming ability of NB4 cells in a dose - dependent manner. Conclusion As4S4 reduces cell viability of NB4 cells in vitro and inhibits the proliferation and colony forming ability of NB4 cell.
出处
《中原医刊》
2006年第12期3-5,共3页
Central Plains Medical Journal
关键词
四硫化四砷
增殖
NB4细胞
Tetra - arsenic tetra - sulfide
Proliferation
NB4 cell
