摘要
目的:建立心可宁胶囊(蟾酥等)中华蟾酥毒基和脂蟾毒配基的含量测定方法。方法:采用HPLC法进行含量测定。色谱柱:十八烷基硅烷键合硅胶为填充剂,流动相:甲醇-水(50∶40),流速:0.8 mL/m in,检测波长:296 nm;柱温:室温。结果:线性范围:华蟾酥毒基3.5μg^0.28μg,脂蟾毒配基3.75μg^0.30μg。平均回收率:华蟾酥毒基为99.86%,RSD为1.8%(n=5),脂蟾毒配基为99.84%,RSD为1.2%(n=5)。结论:该方法简便、灵敏、准确、专属强,能够控制产品质量。
AIM： To establish the method of determining cinobufagin and resibufogenin in Xinkening Capsules （Venenum Bufonis, etc. ）. METHODS： HPLC was applied to determining cinobufagin and resibufogenin. The determination was carried out using an ODS column with a solvent system： methanol-water （50： 40）. The flow rate was 1.0 mL/min, detection wavelength was at 296 nm. RESULTS ： The linear ranges of cinobufagin and resibufogenin were 3.5 μg-0.28 μg and 3.75 μg-0.30 μg, respectively. The average recoveries of cinobufagin and resibu- fogenin were 99.86% （ RSD = 1.8%, n = 5 ）, and 99.84% （ RSD = 1.2%, n = 5 ）, respectively. CONCLUSION ： This method is simple, accurate with strong specificity and can be used to control the quality of Xinkening Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2006年第6期812-815,共4页
Chinese Traditional Patent Medicine
