摘要
对用复合酶酶解扇贝加工废弃物制备水解蛋白的工艺进行了研究,通过正交试验方法确定了用两种复合酶酶解的最适水解条件。结果表明,用枯草杆菌中性蛋白酶和风味酶复合酶酶解的最佳酶解参数为:加酶量比为2∶1,温度为65℃,pH为7.0,时间为5 h,水解液氨基酸态氮含量为50.3%,蛋白质水解度为95.5%。用木瓜蛋白酶和风味酶复合酶酶解的最佳酶解参数为:加酶量比为2∶1,温度为65℃,pH为7.0,时间为5 h,水解液氨基酸态氮含量为43.2%,蛋白质水解度为85.0%。用SephadexG-15葡聚糖凝胶柱层析法对水解产物的分析结果表明,两种混合酶的酶解产物为蛋白肽和游离氨基酸,其相对分子质量均集中分布在585和150左右。
The enzymatic hydrolysis of protein from processed scallop disposal was studied using two groups of proteases through the orthogonal tests. The optimum parameters for the mixture of two groups of proteases were： the enzymatic ratio of neutral proteinase to flavourzyme 2： 1, a temperature of 65℃, pH 7.0 and 5 hours for the mixed proteases of neutral protease and flavourzyme. The hydrolysis results under the optimum conditions showed that content of amino nitrogen in the hydrolytes was 50.8% and the degree of protein hydrolyzing in the hydrolytes was 95.8%. The optimum parameters for another mixed proteases were： the enzymatic ratio of papain to flavourzyme 2： 1, a temperature of 65℃, pH 7.0, and 5 hours. The content of amino nitrogen and the degree of protein hydrolyzed under the optimum conditions were 43.7% and 85.7%, respectively. The molecular distributions of the peptides in the hydrolytes analyzed by Sephadex G - 15 resin indicated that the peptide molecular weights hydrolyzed by two groups of mixed proteases were about 585 Da and 589 Da, respectively. These findings illustrated that the peptides in hydrolytes were mostly small molecular peptides,and can be further processed as new products of hydrolyzed protein.
出处
《大连水产学院学报》
CSCD
北大核心
2006年第2期149-152,共4页
Journal of Dalian Fisheries University
基金
辽宁省自然科学基金项目资助(20022102)
关键词
扇贝加工废弃物
酶解
分子量分布
processed scallop disposal
enzymatic hydrolysis
molecular weight distribution
