期刊文献+

冷藏条件下鲢鱼肌肉蛋白的变化 被引量:13

Changes of silver carp muscle proteins during chilled storage
下载PDF
导出
摘要 利用聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹法分析比较了鲢鱼在0和4℃下冷藏14 d过程中肌肉蛋白的变化情况.结果表明:在2种温度下冷藏,肌浆蛋白降解均不显著,其变化趋势基本一致;肌球蛋白重链在0℃下冷藏14 d,4℃下冷藏11 d后发生明显降解;α-辅肌动蛋白和肌动蛋白在2种温度下冷藏均呈逐渐降解的趋势,且在4℃下冷藏降解更为显著;原肌球蛋白在0和4℃下冷藏2 d后均开始降解,此后于0℃冷藏蛋白无明显变化,但于4℃冷藏11 d后降解速度加快. The changes of silver carp muscular proteins during chilled storage at 0℃ and 4℃ for 14 days were studied using SDSPAGE and western blotting. The result showed that the degradation tendency of sarcoplasmic protein was not obvious and comparable under these two temperatures. Among myofibrillar proteins, obvious degradation of myosin heavy chain (MHC) was observed after 14 days at 0℃ and 11 days at 4℃, respectively. Gradual degradation of α-actinin and actin was detected under both temperatures, and it was more obvious at 4℃. The degradation of tropomyosin could be observed after 2 days both at 0℃ and 4℃. Similar to MHC and actin, tropomyosin degraded significantly at 4℃ after 11 days.
出处 《福建农林大学学报(自然科学版)》 CSCD 北大核心 2006年第3期314-318,共5页 Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金 福建省自然科学基金资助项目(C0410036)
关键词 鲢鱼 肌肉蛋白 降解 冷藏 silver carp muscle protein degradation chilled storage
  • 相关文献

参考文献19

  • 1KJAERSGARD I V,JESSEN F.Proteome analysis elucidating post-mortem changes in cod (Gadus morhua) muscle proteins[J].J Agric Food Chem,2003,51(14):3985-3991.
  • 2KOOHMARAIE M,WHIPPLE G,KRETCHMAR D H,et al.Postmortem proteolysis in longissimus muscle from beef,lamb and pork carcases[J].J Anim Sci,1991,69:617-624.
  • 3HUANG J,FORSBERG N E.Role of calpain in skeletal-muscle protein degradation[J].Proc Natl Acad Sci USA,1998,95:12100-12105.
  • 4KOOHMARAIE M.The role of Ca^2+-dependent proteases (calpains) in post mortem proteolysis and meat tenderness[J].Biochimie,1992,74(3):239-245.
  • 5SHIGEMURA Y,ANDO M,TSUKAMASA Y,et al.Correlation of type Ⅴ collagen content with postmortem softening of fish meat during chilled storage[J].Fish Sci,2003,69:842-848.
  • 6SHIGEMURA Y,ANDO M,HARADA K,et al.Possible degradation of type Ⅰ collagen in relation to yellowtail muscle softening during chilled storage[J].Fish Sci,2004,70:703-709.
  • 7RICHARD G T,GREET H G,VALERY F T,et al.Is z-disk degradation responsible for postmortem tenderization?[J].J Anim Sci,1995,73:1351-1367.
  • 8SEKI N,TSUCHIYA H.Extensive changes during storage in carp myofibrillar proteins in relation to fragmentation[J].Nippon Suisan Gakkaishi,1991,57:927-933.
  • 9TSUCHIYA H,KITA S,SEKI N.Postmortem changes in α-actinin and connectin in carp and rainbow-trout muscles[J].Nippon Suisan Gakkaishi,1992,58:793-798.
  • 10MOCHIZUKI S,SATO A.Effect of various killing procedures and storage temperatures on post-mortem changes in the muscle of horse mackerel[J].Nippon Suisan Gakkaishi,1994,60:125-130.

二级参考文献44

  • 1Lauffenburger D A, Horwitz A F. Cell migration: a physically integrated molecular process. Cell, 1996, 84(3): 359 ~ 369
  • 2Viel A. Alpha-actinin and spectrin structures: an unfolding family story. FEBS Lett, 1999, 460(3): 391 ~ 394
  • 3Blanchard A, Ohanian V, Critchley D. The structure and function of alpha-actinin. J Muscle Res Cell Motil, 1989, 10(4): 280 ~ 289
  • 4Tang J, Taylor D W, Taylor K A. The three-dimensional structure of alpha-actinin obtained by cryoelectron microscopy suggests a model for Ca2+ -dependent actin binding. J Mol Biol, 2001, 310(4): 845 ~858
  • 5Meyer R K, Aebi U. Bundling of actin filaments by alpha-actinin depends on its molecular length. J Cell Biol, 1990, 110(6): 2013 ~2024
  • 6Djinovic-Carugo K, Gautel M, Ylanne J, Young P. The spectrin repeat: a structural platform for cytoskeletal protein assemblies. FEBS Lett, 2002, 513(1): 119 ~ 123
  • 7Ylanne J, Scheffzek K, Young P, Saraste M. Crystal structure of the alpha-actinin rod: four spectrin repeats forming a thight dimer. Cell Mol Biol Lett, 2001, 6(2): 234
  • 8Dixson J D, Forstner M J, Garcia D M. The alpha-actinin gene family:a revised classification. J Mol Evol, 2003, 56( 1 ): 1 ~ 10
  • 9Millake D B, Blanchard A D, Patel B, Critchley D R. The cDNA sequence of a human placental alpha-actinin. Nucleic Acids Res,1989, 17(16): 6725
  • 10Youssoufian H, McAfee M, Kwiatkowski D J. Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am J Hum Genet, 1990, 47( 1 ): 62 ~ 71

共引文献8

同被引文献188

引证文献13

二级引证文献101

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部