摘要
胰源性１４×１０￣３ｕ磷脂酶Ａ＿２（ＰＬＡ＿２）在体外同大鼠中性粒细胞（ＰＭＮ）培养６０ｍｉｎ后，细胞对ＴＮＦ趋化增强，培养１０ｍｉｎ后上清液中血栓素（ＴＸＢ＿２）含量比正常增高（Ｐ＜０．０１）。前列环素（ＰＧＩ＿２）含量不变。ＰＬＡ＿２激动剂Ａ２３１８７也能显著加强中性粒细胞对ＴＮＦ的趋化，并伴有ＴＸＢ＿２释放增多（Ｐ＜０．０１）。此外，ＰＬＡ＿２和Ａ２３１８７还显著增强ＰＭＮ对玻璃珠的粘附活性。使用ＰＬＡ＿２抑制剂二溴苯乙酮（ＰＢＰＢ）和ＰＬＡ＿２多克隆抗体可抑制外源性ＰＬＡ＿２对ＰＭＮ趋化和粘附的增强作用，但对Ａ２３１８７的调节作用无效。以上结果表明ＰＬＡ＿２激活及其代谢产物可能介导ＰＭＮ趋化和粘附作用。
Rat PMN were preincubated with purified pancreatic 14×10￣3u PLA_2(100ng/ml)and cal-cium ionophore A23187 (10nmol/L)for 60 min to investigate the effects of PLA,on PMNchemotaxis and adherence.The enhancement of chemotaxis towards TNF in agarose and in-crease in release of TXB_2 from PMN were observed,Preincubation of PMN with arachidonicacid(AA),a PLA_2 metabolite,also resulted in TXB_2 release.In addition,PLA_2 and A23187also obviously enhanced PMN adhesion to glass beads(2mm).P-bromophenacyl bromide(PBPB),an inhibitor of PLA_2,at the concentration of 5μmol/L blocked the enhancing effectof PLA_2 and A23187,while 10μmol/L AA could reverse the effect of PBPB.The antiserum ofpancreatic PLA_2 had significant inhibitory effect on PLA_2-primed PMN chemotaxis and adher-ence, but no effect on A23187-primed PMN activation.The results indicate that PLA_2activation may promote or prime the chemotaxis and adherence of PMN by the generation of AAmetabolites.
出处
《中国应用生理学杂志》
CAS
CSCD
1996年第1期67-71,共5页
Chinese Journal of Applied Physiology
