摘要
目的:探讨高住高练低训(HiHiLo)对红细胞调节T淋巴细胞免疫能力的影响机制。方法:以16名北京体育大学足球专项运动员为对象,实验组(HiHiLo组)和对照组(低住低训组,LoLo组)各8名。实验组每晚入住低氧房(O2浓度14.2%,相当于海拔3000米高度)10小时,每周在低氧房内进行2次72%最大摄氧量蹬功率自行车30分钟训练;对照组平原居住,每周进行2次平原80%最大摄氧量蹬功率自行车30分钟训练。两组每周进行3次由同一教练执教的专项训练。实验期4周。分别于实验前、入住10小时后、实验2、3、4周末清晨取静脉血,与相应的荧光标记抗体反应,采用流式细胞仪记录红细胞CD58、CD59平均荧光强度及T淋巴细胞CD2阳性细胞率。结果:4周实验后,实验组和对照组CD58表达分别较实验前下降了33.14%和30.29%(均P<0.01),两组间有显著性差异(P<0.05);CD59表达分别下降了10.38%和9.68%(均P<0.01),两组间无显著性差异(P>0.05);T淋巴细胞CD2阳性率在实验期无明显变化,两组间也未见显著性差异(P>0.05)。结果表明,HiHiLo和LoLo均可影响人体红细胞CD58、CD59表达,但HiHiLo的影响作用有更明显的趋势,而T淋巴细胞CD2表达受低氧和/或训练刺激的影响不明显。
To determine the effect of erythrocyte on the modulation of T lymphocyte immune function in athletes during “living high-exercising high-training low” (HiHiLo), sixteen male college soccer players were divided into two groups randomly: HiHiLo group(n=8) trained at sea level and lived in hypoxic chamber with 14.2% of O_2 (simulated altitude of 3,000m) for 28 days,10h/day, while control group (n=8) trained and lived at sea level (LoLo). Additionally, HiHiLo group exercised on the cycle ergometer at 72%VO_2max intensity in hypoxic rooms (30min/time,2 times/week), but control group exercised on the cycle ergometer at 80% VO_2max intensity at sea level. Although the effect on CD58 and CD59 of erythrocyte occurred in HiHiLo group and LoLo group, there was a significant influence on CD58 in HiHiLo group. CD2 of T lymphocyte was not impacted by hypoxia and/or exercise because of its lower sensitivity to hypoxia and/or exercise than erythrocyte.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2006年第3期320-322,340,共4页
Chinese Journal of Sports Medicine
基金
国家自然科学基金项目(批号:30370689)资助