摘要
用基因枪、超声波和子房注射法转化玉米,所用质粒pB48.415带有3＇端截短的Bt毒蛋白基因和潮霉素磷酸转移酶(hpt)基因。用基因枪轰击玉米胚性愈伤组织和幼胚,超声波处理玉米胚性愈伤组织,用自制的微玻针注射授粉后10～20h的玉米子房,均已成功地获得了转Bt基因的玉米植株。点杂交和Southern吸印杂交的结果都证明在转基因玉米植株中存在Bt毒蛋白基因。
Studies on maize transformation have been carried out through bombarding by particle gun, ultrasonicating in a DNA buffer or ovary injecting by a self-made mi-croinjector. The plasmid pB48.415, which carries a 3'-end truncated Bt-toxin protein gene and a hygromycin phosphotransferase (hpt) gene, was used in the transformation. Transgenic maize plants were obtained from immature embryos and embryogenic calli bombarded with particle gun, embryogenic calli ultrasonicated under different conditions or ovaries injected after 10-20h of pollination. The results of Dot blotting and Southern blotting analysis certified the integration of Bt gene into maize genome.
出处
《生物工程学报》
CAS
CSCD
北大核心
1996年第1期45-49,共5页
Chinese Journal of Biotechnology
基金
863项目基金
关键词
玉米
基因转移
转基因植株
Maize, transformation, particle gun, ultrasonication, ovary injection, Bt gene
