摘要
抗人Ｃｌｑ单克隆抗体轻链可变区基因的克隆及序列测定陈克敏，朱锡华（第三军医大学免疫学教研室，重庆６３００３８）目前国内外对基因工程抗体的研究较多，但尚未见补体基因工程的研究报道．一般的方法是经ＲＮＡ反转录合成ｃＤＮＡ，再以ＰＣＲ扩增，克隆Ｉｇ可变区基...
Although the PCR technique originally described by Orlandi allows the specific amplification of immunoglobulin variable genes from cDNA of mouse hybridoma cell lines using primers derived from FR_1 and FR_4 consensing sequences, in this experiment. instead of cDNA, the genetic elements encoding the VJL region of a murine monoclonal antibody against human Clq,namely ClqVL was generated from genomic DNA, cloned and sequenced. The results showed that the VJL-sequence of the Clq with 333 nucleotides belongs to mouse V_k V gene family, resulted from V_k-J_(k5) rearrangment. The information involved in Clq V_L is important for the understanding of Clq lunction and of the pathogenetic and immunological aspects of autoimmune disease.
基金
国家自然科学基金
