摘要
在兔源肠致病性大肠杆菌WF-03-B3菌株和兔源A型魏氏梭菌XT-04-X5菌株原生质体融合的研究中,为了对融合子进行选择鉴定,在呼吸缺陷选择的基础上WF-03-B3菌株02r(氧气抗性);XT-04-X5菌株02s(氧气敏感性),再应用定性和定量药物敏感性试验,对两种菌原生质体融合的耐药性遗传标记进行了筛选.结果表明,WF-03-B3菌株对丁胺卡那霉素AKNs(丁胺卡那霉素敏感);XT-04-X5菌株对丁胺卡那霉素AKNr(丁胺卡那霉素抗性),在此基础上,测定了丁胺卡那霉素在选择培养基中的适用浓度。筛选出兔源肠致病性大肠杆菌WF-03-B3菌株的遗传标记为(AKNs,02r)和兔源A型魏氏梭菌XT-04-X5菌株的遗传标记为(AKNr,02s)。
In order to select and determine fusants of Enteropathogenie Escherichia Coli （O2 resistance, O2^s） and type A Clostridium perfringens （O2 sensitive, O2^s） isolates originated from rabbits, the qualitative and quantitative antimicrobial susceptibility test was used to screen the genetic markers of protoplast fusion between the se2 bacterria. The results show that Enteropathogenie Escherichia Coli （O2 resistance, O2^r） was tolerant to AKN ; type A Clostridium perfringens（O2 sensitive, O2^s） was susceptible to AKN . Based on it, the practical concentrotions of the two antimicrobes in the selective medium plate are determined. Therefore the genetic markers of Escherichia Coli（O2 resistance, O2^r） and type A Clostridium perfringens（O2 sensitive, O2^s） are screened. They are WF-03-B3 （Amikacin Sulfate sensitive, O2 resistance; AKN^a, O2^r）and XT- 04-X5（Amikacin Sulfate resistance, O2 sensitive; AKN^r, O2^s）.
出处
《家畜生态学报》
2006年第2期59-62,共4页
Journal of Domestic Animal Ecology
关键词
肠致病性大肠杆菌
A型魏氏梭菌
原生质体融合
药物敏感性试验
遗传标记
Enteropathogenie Escherichia Coli
type A Clostridium perfringens
protoplast fusion
antimicroblal susceptibility test
genetic marker
