摘要
目的:观察大黄素对急性坏死型胰腺炎(ANP)大鼠全身炎症反应时血浆细胞因子白介素-1β(IL-1β)、TNF-α、IL-10表达的影响。方法:大鼠通过胰腺被膜下均匀注射3%牛磺胆酸钠制备ANP模型,小肠内灌注大黄素25mg/kg和5mg/kg,术后12、24、48h采血5ml,观察血浆IL-1β、TNF-α和IL-10表达情况。结果:ANP组淀粉酶较正常组高,且随时间而加重;在各时间点,大黄素治疗组淀粉酶较ANP模型组明显下降;ANP组血浆IL-1β、TNF-α、IL-10较正常组高,大黄素治疗组可见IL-1β、TNF-α、IL-10较ANP模型组明显下降。结论:大黄素减轻急性坏死型胰腺炎大鼠全身炎症反应时的多器官损伤的机制可能是大黄素负性调节细胞因子IL-1β、TNF-α和IL-10的表达。
Introduction： Studies have revealed that emodin is a potent agent in the management of clinical and experimental acute pancreatitis, but the molecular mechanisms by which emodin produces its biologic effects remain unknown. Objectives： To investigate mechanisms of the Chinese herb emodin in acute necrotic pancreatitis of rats during systemic inflammatory response syndrome by analyzing the changes of the plasma level of interleukin-1β （IL-1β）, TNF-α, IL-10. Methods： SD rats were divided into four groups randomly： normal control group, ANP（acute necrotic pancreatitis） model without emodin treatment group and ANP model with emodin treatment group. Acute necrotic pancreafitis was induced by injection of 3% sodium taurocholic acid into the subcapsular of pancreas. Emodin （5mg/kg and 25mg/kg） was administered by injecting into the intestines. Rats were killed at 12, 24 and 48 hours after the operation. Plasm amylase was measured by means of enzymatic assay with spectrophotometer. The plasma level of IL-1β, TNF-α and IL-10 were evaluated by radioimmunoassay at 12, 24 and 48 hours following acute necrotic pancreatitis. Results： The plasma amylase decreased significantly in the emodin-treated group in comparison with non-treated group. The plasma level of IL-1β and TNF-α were decreased significantly in the emodin-treated group（25mg/kg） in comparison with non-treated group. IL-10 was increased significantly in the emodin-treated group in comparison with non-treated group. Conclusion： The Chinese herb emodin can ameliorate the systemic inflammatory response during acute necrotic pancreatitis. The mechanisms might be ascribed to its negative modulation of IL-1β, TNF-α and up-regulation of IL-10.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第2期15-16,共2页
Pharmacology and Clinics of Chinese Materia Medica
