摘要
目的:研究玉屏风散及其所含多糖成分对小鼠免疫功能的影响。方法:以环磷酰胺造成免疫低下小鼠模型,通过腹腔巨噬细胞吞噬试验研究药物对非特异性免疫的影响;通过溶血素抗体法研究受试药物对体液免疫的影响;通过DNCB所致的迟发型超敏反应研究药物对细胞免疫的影响;免疫荧光染色法测定外周血中CD4+和CD8+T细胞的比例,ELISA测定脾细胞IL2分泌和呼吸道、肠道灌流液中IgA含量。结果:玉屏风散总提取物及多糖对各实验指标均有显著增强作用;黄芪对各指标亦有一定增强作用,其强度弱于全方;防风显著促进肠道和呼吸道IgA分泌,而对细胞免疫无影响;白术仅增强巨噬细胞的吞噬功能;全方及各组成药物总提物和多糖的作用无显著性差异。结论:多糖成分是玉屏风散免疫干预作用的主要有效部位。
Objective： To study the effect of Yupingfengsan（YPFS） and its polysaccharides on mice immunity function. Methods： Use cyclophosphamide to setup the low immunity mice model, and study the effect of the drug on macrophage phagocytosis, hemolysin- antibody product, delayed hypersensitive reaction induced by DNCB. Determine the proportion of CD4^＋ and CD8^＋ T cell by immunofluorescence. Use ELISA to determine the IgA content and the IL-2 secretion in spleen cell. Results： YPFS could enhance those indexes; Huangqi could also enhance thosee indexes but weaker than YPFS; Fangfeng could increase the IgA secretion but had no effect on cellular immunity; Baichu could only enhance the macrophage phagocytosis; YPFS and its total extract had no prominent difference with the polysaccharide on immunity. Conclusion： The polysaccharide was the main effective immuno- intervention component in YPFS.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第1期2-4,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
四川省中医药管理局资助项目(2003A38)
四川省科技厅基础项目(04JY0290056)
