摘要
目的探讨外周血淋巴细胞(PBL)穿孔素和颗粒酶B表达水平在肾移植诊断急性排斥反应(AR)和抗排斥疗效中的关系。方法采用定量逆转录PCR(RT-PCR)方法动态测定AR(n=7)、肾功延迟恢复(n=8)、近期肾功正常(n=27)、长期肾功稳定(n=25)组67例肾移植患者移植前后PBL穿孔素和颗粒酶B表达水平和AR的关系。结果肾移植术后患者PBL穿孔素和颗粒酶B表达强度依次为AR组、肾功延迟恢复组、近期肾功正常组、长期肾功稳定组,AR组与其它3组差异有统计学意义(P<0.01);其升高时间比临床上出现AR的症状早约3d,随着AR的逆转,其表达也逐渐降至原有基础水平。结论定量RT-PCR测定PBL穿孔素和颗粒酶B的表达可以是一种无创的、较敏感的早期诊断肾移植AR的方法,并可预测抗排斥反应治疗效果。
Objective To investigate the effect of perforin and granzyme B in peripheral blood lymphocytes during acute rejection in renal transplantation. Methods Sixty - seven recipients of renal allograft were involved in the study. The recipients were divided into four groups ： group I with 7 cases of acute rejection, group 2 with 8 cases of delayed graft function, group 3 with 27 cases of stable function and group 4 wih 25 cases of long-term survival. The expressions of perforin and granzyme B of peripheral blood lymphocytes were studied by quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results The expressions of perforin and granzyme B in acute rejection group were significantly higher than those of the other three groups （ P 〈0.01 ）. The expressions of perforin and granzyme B were increased distinctly in those who had acute rejection, and the time was about 3 days earlier than the appearance of clinical symptoms. Conclusion The analysis of expression of perforin and granzyme B by quantitative RT-PCR may be used as a non-invasive and sensitive method to diagnose the acute rejection and monitor the effect of antirejection therapy.
出处
《中国医师杂志》
CAS
2006年第6期741-743,共3页
Journal of Chinese Physician
基金
广东省深圳市科技局立项项目资助(200404083)
关键词
肾移植
移植物排斥
膜糖蛋白类
丝氨酸内肽酶类
淋巴细胞
Kidney transplantation
Graft rejection
Membrane glycoproteins
Serine endopeptidases
Lymphocytes
