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醇化烟叶酵母菌分离鉴定及其生物学特性研究 被引量:8

Isolation and identification of yeast in aging tobacco leaves and its biological characteristics
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摘要 采用涂布培养法和富集培养法对醇化烟叶样品中酵母菌进行了分离,富集培养法分离到23株,涂布培养法分离到6株。29株酵母菌的生物学特性研究表明,其中果胶酶、蛋白酶、淀粉酶、脂酶、纤维素酶、酚类利用、木质素利用、尼古丁降解、发酵产物气味等试验呈阳性的菌株数分别是1、10、0、3、10、22、1、21和29株。利用ATB系统,将ATY1、ATY8和ATY27等3株酵母菌分别鉴定为黏红酵母(Rhodotorula glutinis)、罗伦隐球酵母(Cryptococcus laurentii)和粗壮假丝酵母(Candida valida),各菌株鉴定结果的符合率分别为99.4%、98.2%和89.9%。研究结果表明,醇化烟叶中酵母菌种群数量较小,但酵母菌的生物学特性有利于烟叶醇化,值得进一步深入研究。 The yeasts in aging tobacco leaves were isolated by spread plate method and enrichment cultivation method. Twenty three strains of yeasts were obtained from different samples by enrichment cultivation method and six strains of yeasts were obtained by spread plate method. The biological characteristics of the twenty nine strains of yeasts were investigated. The results showed that the number of positive reaction of pectinase, proteinase, amylase, esterase, cellulase, ligninolysis, polyphenol conversion, degradation of nicotine and fragrance test were 1, 10, 0, 3, 10, 22, 1, 21 and 29. By ATB system, the yeasts strains ATY1, ATY8 and ATY27 were identified as Rhodotorula glutinis, Cryptococcus laurentii, Candida valida, and their identification rate were 99.4 %, 98.2 % and 89.9 %. The results showed that the amount of yeasts population is less in aging tobacco leaves, but the biological characteristics of the yeasts are propitious to tobacco leaf aging and worthy of further study.
作者 祝明亮
出处 《工业微生物》 CAS CSCD 北大核心 2006年第2期22-25,共4页 Industrial Microbiology
基金 国家烟草专卖局科技计划资助项目(110200202004B)
关键词 醇化烟叶 酵母菌 分离 鉴定 生物学特性 aging tobacco leaves yeast isolation identification biological characteristics
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  • 1顾金刚,方敦煌,李天飞,刘杏忠.防治烟草黑胫病的根际细菌分离与筛选[J].中国烟草学报,2001,7(3):19-22. 被引量:17
  • 2刘石,余永茂,高芳馨,张淑华,马元生.Cu^(2+)在烤烟发酵中对多酚氧化酶的作用[J].烟草科技,1989,22(5):22-23. 被引量:10
  • 3郭俊成,程晓蕾,肖厚荣,黄文川,倪瑞.皖南烤烟陈化研究[J].中国烟草,1996,17(2):16-17. 被引量:35
  • 4余永茂 高芳馨.微生物酶发酵低次烟叶初试报告[J].烟草科技,1988,(5):16-18,29.
  • 5余校芳 国家烟草专卖局科技教育局.烟叶最佳储存期研究.烟草科学技术成果汇编第三辑(1993-1995)[M].郑州:河南科学技术出版社,1996.55.
  • 6张广周.卷烟降焦·烟叶贮存·叶面快速估测新法简介[J].四川烟草,1997,(5):47-47.
  • 7罗家基 朱子高 等.微生物的烟中叶发酵过程中的作用[J].烟草科技,1998,(1):6-6.
  • 8中国烟草总公司.烟草工业实用技术手册[M].贵阳:贵州人民出版社,1989.154-176,216.
  • 9徐洁 张修国 郑小嘎 等.烤烟诱香微生物及其制剂的研究初选.云南烟草,2003,(1):25-25.
  • 10王革 祝明亮 韦建福 等.烟叶中有益醇化微生物的分离和筛选.烟草科学研究,2000,(4):45-45,55.

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