摘要
用基质辅助激光解吸附飞行时间质谱（ＭＡＬＤＩ－ＴＯＦ－ＭＳ）法分析测定了人血清白蛋白。结果表明，全军血液制品研究中心应用Ｒｉｖａｎｏｌ和Ｃｏｈｎ＇ｓ两种方法分离提取的人血清白蛋白，其分子量与理论计算值相吻合，其纯度与市售标准人血清白蛋白相一致，用ＭＡＬＤＩ－ＴＯＦ－ＭＳ法分析鉴定人血清白蛋白，准确、简便和灵敏（仅需ＰＭ浓度样品），且重复性好，日内和日间差的变异系数均小于０．０３％，是ＳＤＳ－ＰＡＧＥ电泳法所无法比拟的。
The matrix-assisted UV laser desorption ionization time of flight mass sperctra are reported for human albumin which isolated from healthy human blood by Rivanol and Cohn's methods.α-cyano-4-hydroxycinnamic acid is used as matrix in analysis. The RSD within day and between days for the molecular mass determination of human albumin are less than 0. 03 %. The experimental results indicate that MALDI-TOF-MS can be used as a routine method for characterization of human albumin.
出处
《分析测试学报》
CAS
CSCD
1996年第4期80-82,共3页
Journal of Instrumental Analysis
