针刺预处理对脑缺血再灌注大鼠中枢杏仁核CRF样蛋白表达的影响被引量：3

下载PDF

导出

摘要目的:采用免疫组织化学技术,探讨肾俞、百会穴针刺预处理对脑缺血再灌注后不同时间段大鼠中枢杏仁核CRF的表达的影响。方法:雄性Wistar大鼠130只,随机分成5组;以双侧肾俞、百会穴电刺激为针刺预处理方式,以颈动脉引流法行全脑缺血再灌注造模;脑组织恒冷箱连续冠状切片,免疫细胞化学ABC反应,图像分析系统行杏仁中央核群促肾上腺皮质激素释放因子样阳性免疫面积、平均吸光度检测。结果:各组CRF表达在不同时间段出现显著性变化。术后/针后6h,与空白对照比较,各组CRF表达显著升高(P<0.05);术后/针后24h,除空白对照组外各组CRF表达均出现明显回落,组间仍存在显著差异(P<0.05);术后/针后72h,脑缺血再灌注组及针刺预处理脑缺血再灌组CRF表达仍高于正常水平且两组间差异明显(P<0.05),而针刺预处理对照组及假手术对照组均已回落至正常水平(P>0.05)。结论:脑缺血再灌注损伤可导致中枢杏仁核CRF样蛋白的强烈表达;肾俞、百会穴针刺预处理对脑缺血再灌注所致的中枢杏仁核CRF的异常表达具有明显的调控作用。

作者陈泽斌柯晖邹锋

机构地区湖北中医学院

出处《中医药学报》 CAS 2006年第3期14-16,共3页 Acta Chinese Medicine and Pharmacology

基金湖北省自然科学基金资助项目(2005ABA188)

关键词脑缺血再灌注损伤杏仁核促肾上腺皮质激素释放因子针刺预处理

分类号 R245.31 [医药卫生—针灸推拿学]

引文网络
相关文献

参考文献13

1Bale TL, Giordano FJ, Hickey RP, et al. Corticotropin - releasing factor receptor 2 is a tonic suppressor of vascularization[J]. Proc Natl Acad Sci U S A,2002,99(11 ) :7734 - 7739.
2Sapolsky RM. Giueocorticoids hippocampal damage and the glutamatergic synapse[J] .Prog Brain Res,1990,86:13-23.
3陈俊峰,蒋豪,李煊.糖皮质激素加剧缺血诱导神经细胞损害的研究[J].中华麻醉学杂志,2000,20(10):628-629. 被引量：8
4Tczval H,Jahn O,Todorovic C,et al. Cortagine,a specific agonist of corticotropin - releasing factor receptor subtype 1, is anxiogenic and antidepressive in the mouse model[J].Proc Natl Acad Sci U S A,2004,101 (25):9468 - 9473.
5Hyman SE. Brain neurocircuitry of anxiety and fear: Implications for clinical research and practice[J]. Biol psychiatry, 1998,44:1201 - 1212.
6Tringali G Aubry JM, Moscianese k, et al. Valproic acid inhibits corticotropin - releasing factor synthesis and release from the rat hypothalamus in vitro:evidence for the involvement of GABAergic neurotransmission[J].Psychiatry Neuresci, 2004, 29(6) :459 - 466.
7Goldstein LE, Rasmusson AM, Bunney BS, et al. Role of the amygdalain the coordination of behavioral, neuroendocrine, and prefrontal cortical monoamine responses to psychological stress in the rat[J]. Neuroscience,1996,16:4787 - 4798.
8陈泽斌,王华.大鼠穴位针刺预处理抗脑缺血再灌注损伤作用探讨[J].针刺研究,2003,28(3):165-169. 被引量：34
9Swanson LW, Petrovich GD.What is the amygdala? [J] .Trends Neurosei,1998,21:323 - 331.
10Bohus B, Konlhass JM,Korte SM,et al.Forebrain pathways and their behavioral interactions with neuroendocrine and cardiovascular function in the rat[J]. Clinical and Expeimental pharmacology and physio logy,1996,23:177- 182.

二级参考文献12

1应赛霞,程介士.电针对沙鼠急性脑缺血再灌注后神经原损伤的保护作用[J].神经科学,1994,1(1):33-36. 被引量：18
2罗勇,黄仲荪,徐晨.针刺对缺血性脑梗塞大鼠皮层神经元线粒体影响的形态计量研究[J].上海针灸杂志,1996,15(6):28-30. 被引量：29
3夏萤曹小定.针刺对中枢递质的影响[J].针刺研究,1985,10(2):81-81.
4Barone FC,Corbett D. Ischemic preconditioning in 18-to 20-month-old gerbils: long-term survival with functional outcomeme asures. Stroke, 1999,30(6) : 1240.
5Stagliano NE, Perez-pinzon MA, Moskowitz MA, et al. Focal ischemic preconditioning induces rapid tolerance to middle cerebral artery occlusion in mice. J Cereb Blood Flow Metab,1999,380(2) :91.
6Bannister CM, Sonia A. Isocheimal andrevascularization of the middle cerebral territory of the rat brain by manipalation of the blood vessels in the neck. Surg Neurol, 1984,21:351.
7Pulsinelli WA. Selective neuronal vulnerability: Morphlogical and molecular characteristics. Prog Brain Res, 1992,581 : 168.
8Kirino T.Delayed neuronal death in the gerbil hippocampus following isocheimal Brain Res.1982.239:57.
9Chen J F，Eur J Pharmacol，1998年，347卷，67页
10陈泽斌,韩永明,熊迎春,吴昌清,袁尚荣.针刺大鼠“肾俞”穴诱导c-fos原癌基因在下丘脑室旁核中的表达[J].针刺研究,1999,24(3):187-191. 被引量：9