摘要
从斑点叉尾鱼回鱼皮中提取酸溶和酶溶胶原并对其进行了部分定性研究。实验中获得的胶原中甘氨酸最为丰富,质量分数超过23%,酸溶胶原的变性温度为32.5℃,比猪皮胶原的变性温度低5℃。SDS-PAGE实验结果表明酸溶胶原至少有2条不同的α链。酸溶胶原和酶溶胶原分别占原料干基的25.8%和38.4%,可以作为猪皮、牛皮胶原的替代品。
Acid-soluble and pepsin-soluble collagens （ASC and PSC） were extracted from the skin of Channel catfish and partially characterized. The collagen obtained in the experiment contained more than 23% glycine and it is the most abundant amino acid. The denaturation temperature of acid-soluble collagen from the Channel catfish was 32.5℃ ,about 5℃ lower than that of the porcine skin collagen. SDSPAGE showed that the collagens were composed of two distinct alpha chains. The ASC and PSC, on a dry weight basis, were 25.8 % and 38.4%, respectively. These results suggest that Channel catfish skin has potential as a supplement of the source of vertebrates collagen.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第3期342-345,350,共5页
Journal of Jilin Agricultural University
基金
教育部食品科学与安全重点实验室资助项目(教食安04005)
关键词
斑点叉尾鱼回
胶原
氨基酸
变性温度
Channel catfish
collagen
amino acid
denaturation temperature
