摘要
为了全面正确地认识和评价畜禽饲料中农药残留状况,保证畜禽产品食用的安全性,利用弹性石英毛细管气相色谱分析技术,研究了7种氨基甲酸酯类农药在饲料中的多残留分析方法.结果表明,饲料样品中的氨基甲酸酯类农药以丙酮提取,经等体积石油醚/二氯甲烷混合液萃取,再经弗罗里硅土柱净化,用配有氮磷检测器的毛细管气相色谱仪检测,可以获得满意结果.当添加0.1,0.5,5.0mg/kg时,添加回收率为79.00%～102.37%,变异系数为0.99%～10.03%;方法最小检出量为4.086×10-11～9.324×10-11g,最小检出量为0.02～0.04mg/kg.该方法能一次同时检测饲料中残留的7种氨基甲酸酯类农药,具有高效、简便、快速的特点,可极大地提高工作效率.
In order to recognize and evaluate the residue level of insecticide completely and correctly, and assure the security of animal food consumption. A multiresidue method which can be used to analyze seven carbamate insecticides （metolcarb, isoprocarb, fenobucarb, bendiocarb, carbofuran, pirimicarb, carbaryl）simultaneously in animal feed was established using capillary gas chromatography with nitrogen-phosphorus detection. The result showed that the residue of seven carbamate insecticides in feed sample was extracted with acetone, and the extract was re-extracted with petroleum ether/Dichloro methane. After being concentrated by evaporation, the re-extract were cleaned up by a column chromatography on florisil. The final residue can be detected by GC-NPD with high sensitivity. The average recoveries from untreated control samples fortified with the seven carbamate insecticides at levels of 0.1, 0.5 and 5.0 mg/kg ranged from 79.00% to 102.37%, and the coefficient of variation was from 0.99% to 10.03%. The lowest detectable quantity was 4.086 × 10^-11 ～9.324 × 10^-11g, and the lowest detectable concentrations was 0.02～0.04 mg/kg. This method can analyze the residue of carbamate insecticide simultaneously. It is not only characterized by simplicity, rapidness and efficiency but also can promote work efficiency.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期336-339,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省农业厅项目(2003018)
湖南省环保局项目(200208)
