摘要
用随机扩增多态DNA(RAPD)技术对神农架地区4个居群28个珙桐(Davidia involucrataBaill.)个体进行了遗传多样性分析.16个10 bp寡核苷酸引物共检测到87个位点,多态位点49个,多态百分率56.3%,而居群平均多态位点百分率为30.2%.用POPGENE对数据进行了分析,结果显示:居群的平均Nei’s基因多样性为0.111 8,Shannon’s遗传多样性信息指数为0.165 1,总的居群基因多样性为0.169 2,基因分化系数为0.339 2.这表明神农架地区珙桐的遗传多样性较低,其遗传变异以居群内遗传变异为主.
Twenty-eight individuals of four Davidia involucrata Baill. populations in Shennongjia area, Hubei Province were analyzed by using random amplified polymorphic DNA （RAPD） markers to determine the genetic variation among and within the populations. Eighty-seven loci were detected using sixteen 10 bp random primers, of which 49 were exhibited polymorphism. The average percentage of polymorphic loci （PPL） was 30.2%, while that of polymorphic loci （PPL） was 56.3%. Data were analyzed by the software POPGENE and results showed that the average Nei＇ s genetic diversity was 0.111 8, Shannon＇ s genetic diversity information index was 0. 165 1, genetic diversity of all populations was 0. 169 2 and Gst was 0.339 2. The results indicate that D. involucrata in this area has relatively low genetic diversity and most of differentiation is within populations.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2006年第3期66-70,共5页
Journal of Beijing Forestry University
基金
湖北省教育厅科学研究计划技术创新项目(2001067001)
三峡大学科学基金重大项目(611202)
关键词
珙桐
遗传多样性
RAPD
Davidia involucrata Baill. , genetic diversity, RAPD
