摘要
对人工合成的SOD模拟物铜（Ⅱ）甘氨酸的毒性及其吸收后血清半衰期进行了初步探索。通过灌胃急性毒性、慢性毒性、遗传毒性和灌胃后吸收入血清的药时变化进行了研究。研究结果表明：此物对小鼠灌胃的LD50为418．79±17．72mg／kg，连续给药1周后，小鼠的心、肝、脾、肺、肾和肠胃等脏器在形状、大小和颜色上与阴性对照组无明显差异；高剂量组给药后小鼠的嗜多染红细胞微核率有一定增长（P〉0．05）；经不同浓度给药后，血清中血药浓度峰值时间为220～400min，半衰期为80～90min。证明此药为中等毒性，适当剂量口服后无明显蓄积毒性，大剂量使用时可导致一定的染色体损伤，可望在植物生长调节剂、医药或化妆品、饲料、食品中的抗氧化添加剂及着色抑菌的防腐添加剂等中代替天然SOD。
For the purpose of examining the toxicity and efficacy of Cu（ Ⅱ）-Gly and providing reference for its applications, acute toxicity, chronic toxicity and polychromatic erythrocyte （PCE） micronucleus tests on mice are conducted. In addition, SOD activity changes in mouse blood are measured after oral exposure. It turns out that LD50 is found to be 418.79± 17.72 mg/kg by oral administration, which falls in the category of medium toxicity according to the acute toxicity classification （WHO, 197）. Consecutively stomach dosing shows no obvious influence on the mice organs and the PCE micronucleus rates （‰） are 2.80 ± 1.81, 2.50 ± 1.71 and 2.00 ± 1.15 respectively. This indicates that under the experimental conditions Cu（ Ⅱ ）-Gly has no cumulative toxicity. High dosage can increase the micronucleus rate（ P 〈0.05）. After the stomach dosing at dosage of 200,150 and 100 mg/kg, the medicine peak time in mice blood appears at 400, 280 and 220 minutes and the serum half-life time is about 80 minutes individually.
出处
《北京联合大学学报》
CAS
2006年第2期17-19,22,共4页
Journal of Beijing Union University
基金
北京市教委科技发展基金项目(00KJ-069)
