摘要
目的探讨GNB3基因C825T多态性与长春地区原发性高血压之间的关系。方法入选原发性高血压患者269例,正常人群229例。所有研究对象常规方法提取白细胞DNA。采用多聚酶链反应结合限制性内切酶(BseDI)方法检测GNB3基因C825T多态性。结果共成功检测268例原发性高血压患者和218例正常人。原发性高血压组与正常对照组之间基因型和等位基因频率无显著性差异(P=0.075,0.845)。各基因型之间收缩压、舒张压、脉压和平均动脉压均没有达到统计学显著性差异(P>0.05)。结论GNB3基因C825T多态性可能与长春地区原发性高血压无关。
Objective To investigate the relationship of GNB3 gene C825T polymorphism and essential hypertension in Changehun region. Methods 269 patients with essential hypertension were enrolled into this study. 229 healthy people were used as control group. DNA was extracted from white blood cell. Segments of GNB3 were amplified from DNA by polymerase chain reaction（PCR）. PCR products were restricted with 2 U of BseDI for one night at 55℃. The digested products were subjected to eleetrophoresis in 3% agarose gels and stained with ethidium bromide. Results 1. We amplified 268 essential hypertensive patients and 218 controls successfully. 2. GNB3 C825T genotype in the eases and controls were CC, CT, TT 17.5%, 49.3%, 33.2% and 22.0%, 39. 0%, 39.0% respectively. C allele frequencies in the eases and controls were 41.5% ： 42.2%. 3. SBP, DBP, pulse pressure and mean arterial pressure were not different in eases and control group （P 〉 0.05）. Conclusion The C825T polymorphism in the GNB3 gene may not be associated with essential hypertension in Changehun region.
出处
《中国微循环》
北大核心
2006年第3期205-207,共3页
Journal of Chinese Microcirculation
