摘要
目的:检测膀胱移行细胞癌(BTCC)中Smad4及TGF-β1表达,分析其与BTCC临床分期、病理分级、转移及复发的关系。方法:采用免疫组织化学方法检测42例BTCC组织Smad4和TGF-β1的表达。结合临床病理资料,分析Smad4,TGF-β1与BTCC的侵袭、血管生成、转移及复发间的关系以及Smad4蛋白表达与TGF-β1表达的相关性。结果:Smad4蛋白阳性表达染色位于细胞浆内,BTCC组中阳性率(33.3%)明显低于对照组(83.3%),且随BTCC临床分期及病理分级的升高而降低,肿瘤复发组低于无复发组,肿瘤转移组低于无转移组(P<0.05)。TGF-β1蛋白在正常膀胱组织与BTCC组织中均有表达,其阳性表达率分别为100%和64.3%(P<0.01)。BTCC中Smad4蛋白与TGF-β1蛋白的表达均下调,且呈正相关(r=0.829,P=0.000)。结论:随肿瘤临床分期的升高、组织学分级的升高及肿瘤的转移复发,BTCC中Smad4蛋白与TGF-β蛋白表达均明显下调。
Objective To determine the expression of Smad4 and TGF-β1 in bladder transitional cell carcinoma （ BTCC ） , and to understand the mechanism of invasion, angiogenesis, and metastasis of BTCC. Methods The expressions of Smad4 and TGF-β1 in samples of 42 human bladder carcinoma and 12 normal bladder mucosa tissues were determined with standard immunohistochemical analysis. We also analyzed the relationship among the expressions of Smad4 and TGF-131 and invasion, angiogenesis, and metastasis of BTCC, and the correlation between Smad4 and TGF-β1. Results The positive rate of Smad4 in BTCC was significantly lower than those in normal bladder mucosa tissues （33.3% vs 83.3% , P 〈 0.01 ）. The expressions of Smad4 in poorly differentiated, invasive, recurrent, or with lymph node metastasis of BTCCs were lower than those in well differen- tiated, superficial, nonrecurrent, or without lymph node metastasis ones （ P 〈 0.05 ）. The positive rate of TGF-β1 in BTCC was significantly lower than that in normal bladder mucosa tissues （64.3% vs 100%, P 〈0.01 ）, which was positively correlated to that of Smad4 （P = 0. 000）. Conclusion The expressions of Smad4 and TGF-β1 in BTCC decrease with the increase in clinical stage, poor pathological grade, and the recurrence and metastasis of BTCC.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第3期363-366,共4页
Journal of Central South University ：Medical Science
