摘要
目的探讨Bag-1及p53的表达与胆管癌发生、发展的关系,评价两者表达的相关性及其临床意义。方法应用免疫组织化学SABC(链霉亲合素-生物素化过氧化物酶反应)法检测45例胆管癌组织Bag-1及p53蛋白的表达情况。结果45例胆管癌组织中Bag-1、p53蛋白表达阳性率分别为62.22%和46.67%;15例胆管炎组织中两基因均微表达或不表达;Bag-1和p53的表达与胆管癌患者的性别、年龄和组织学类型无关;Bag-1和p53的高表达均与胆管癌分化的程度、TNM分期和转移状况密切相关;Bag-1、p53表达呈显著正相关(P=0.0153),相关系数r=0.3614。结论bag-1与胆管癌发生密切相关,可作为胆管癌的早期诊断指标;bag-1和p53的联合检测可能较好反映胆管癌的恶性生物学行为,作为判断其预后的参考指标,为今后胆管癌的基因治疗提供依据。
[Objective] To explore the relationship between the expression of Bag-1 and p53 and in cholangiocarcinomas and to evaluate the correlation and clinical significance between them. [Methods] 45 cases of cholangiocarcinoma were examined for Bag-1 and p53 by the method of SABC immunohistocbemistry. [Results] Positive rates of Bag-1 and p53 immunostaining in cholangiocarcinomas were 62.22% and 46.67%. The two genes did not or little express in 15 cholangitis. The expression of the proteins was not significantly correlated to gender, age and histologieal type. The high expression of the two proteins was significantly correlated to differentiated degree of cholangiocarcinoma, TNM classification and aversion. There was significant positive correlation between Bag-1 and p53, （r=0.3614）. [Conclusion] Bag-1 was significantly correlated with the development of cholangioearcinoma, and could be as an early diagnostic criteria of cholangiocarcinoma. The united detection of Bag-1 and p53 could better reflect the malignant biological behaviour of cholangiocarcinoma, and act as a referred index of prognosis, and provide reference for gene therapy in cholangiocarcinoma in future.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第12期1789-1791,共3页
China Journal of Modern Medicine
