摘要
目的：探讨SDS-PAGE和等电聚焦电泳应用于燕窝蛋白分离及燕窝鉴定中的可行性。方法：提取印尼燕窝、怀集燕窝及常作为伪品的明胶、银耳、猪皮的蛋白质,进行SDS-PAGE和等电聚焦电泳研究。结果：印尼燕窝与怀集燕窝在SDS-PAGE图谱上可见明显差异,而明胶、银耳和猪皮等伪品可见特征性蛋白条带。燕窝蛋白经等电聚焦电泳可见清晰的蛋白条带,且主要集中于酸性端。结论：SDS-PAGE和等电聚焦电泳均可获得燕窝蛋白质的特征性电泳图谱,用于鉴别不同品种燕窝及掺伪燕窝是可行的。
Object： To probe the feasibility of SDS-polyacrylamide gel electrophoresis （SDS-PAGE） and isoelectrofocusing （IEF） in the separation and identification of proteins of Edible Bird＇s Nest （EBN）. Methods： SDS-PAGE was performed with samples including Indonesia EBN, EBN from Huaiji of Guangdong Province and three impure EBN samples which were added with gelatin, white fungus or pork skin respectively. IEF was also performed with Indonesia EBN. Results： On SDS-PAGE patterns, there were obvious differences between Indonesia EBN and Huiji EBN, and clear proteins stripes of three impure EBN were visualized. On IEF pattern, clear proteins stripes which focus on the acidic end were visualized. Conclusions： SDS-PAGE can be used in identification of EBN and IEF can be used to produce characteristic pattern of EBN by separating proteins.
出处
《世界科学技术-中医药现代化》
2006年第3期30-32,90,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
关键词
燕窝
SDS-PAGE
等电聚焦电泳
Edible bird＇s nest
SDS-polyaerylamide gel eleetrophoresis
Isoeleetrofocusing
Electrophoresis
