摘要
为了解桔梗栽培种质、野生种质的遗传背景,为桔梗育种及道地药材研究提供依据,采用RAPD方法分析了桔梗20份栽培种质、3份野生种种质及5份遗传材料共92个样品的遗传多样性。从264个随机引物中筛选出多态性较好的6个引物进行全部样品PCR扩增,得到多态性条带58条。所有种质的多态性位点比率(P)为66.67%,Nei's基因多样性指数Ht为0.2099,栽培种质的Ht和P值AT＞AB＞NC＞SZ,野生和遗传材料中各份种质的Ht和P值很低,但总体值仍高；UPGMA聚类和SPSS聚类可以将各种不同来源的种质很好地区分开来。结论：桔梗栽培种质在遗传背景来上为混杂群体,纯系材料遗传背景均一,不同栽培产区桔梗种质已出现明显遗传分化。
To learn the information about the genetic background of the cultivation and wild germplasms of Platycodon grandiflorum assisting of its breeding and studies on genuine characteristics, the genetic diversity of 92 samples （including 20 cultivation and 3 wild germplasms, and 5 genetic materials） were analyzed by RAPD markers. 6 primers from 264 were selected and applied to the PCR amplification for all samples and 58 polymorphic bands were found. For all germplasms, the percentage of polymorphic loci （P） was 66.7%, and Nei＇s gene diversity index （Ht） was 0.2099. The number of P and Ht among cultivation germplasn were AT〉AB〉NC〉SZ, which of each germplasm of wild and genetic materials were small, but of the population were still big. The dendrogram from the Cluster of UPGMA and SPSS of the RAPD genetic distance showed the good classification of all germplasms. The results showed the genetic background of cultivation gerplasms of Platycodon grandiflorum is diversify, however which of genetic materials is uniform; Genetic differentiation between cultivation gerplasm from different areas is obvious.
出处
《世界科学技术-中医药现代化》
2006年第3期37-41,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家十五科技攻关项目(2004BA721A24):道地药材桔梗种质资源及其评价研究
国家中药材扶持资金专项(2005):黄芩
桔梗品种选育及良种繁育基地建设
北京市科技新星计划(2004A60):A类
关键词
桔梗
种质
遗传多样性
RAPD
Platycodon grandiflorum
germplasm
genetic diversity
RAPD
