摘要
为了研究成纤维细胞生长因子5(FGF5)基因在山羊被毛生长中的的作用,进一步寻找与山羊被毛生长相关的遗传标记,为山羊绒毛性状的标记辅助选择和育种提供一定的理论依据,根据人和小鼠成纤维细胞生长因子5(FGF5)基因的同源序列设计引物对山羊基因组进行PCR扩增,将扩增片段进行克隆和测序,并与人和小鼠的成纤维细胞生长因子5基因序列进行同源性比较,确定扩增的片断为山羊的FGF5基因片断。采用PCR-SSCP技术分析了FGF5基因外显子在内蒙古绒山羊、辽宁绒山羊和文登奶山羊品种的多态性,结果表明:FGF5基因在P1和P2引物扩增片段中存在PCR-SSCP多态性,而在P3和P4中没有发现多态。经克隆测序分析,位于外显子1的引物1内存在A→G突变;引物2中发生了碱基序列C→T的突变,这2个突变位点均没有引起编码氨基酸的改变,属于同义突变。对不同山羊品种的基因型和基因频率统计结果表明,引物1中内蒙古绒山羊、辽宁绒山羊、文登奶山羊均以等位基因B为主,且不同群体均处于Hardy-Weinberg平衡;引物2中内蒙古绒山羊、辽宁绒山羊均以等位基因E为主,而文登奶山羊的基因型频率与其他品种有显著差异;内蒙古绒山羊、辽宁绒山羊在引物2位点的基因频率处于Hardy-Weinberg不平衡状态,而文登奶山羊的基因频率处于Hardy-Weinberg平衡状态。
In order to study the ruction of FGF5 on goat hair growth and to find the genetic marker of goat hair , it was designed and aimed to identify the single nucleotide polymorphisms （SNPs） of FGF5 gene in various goat breeds including Inner Mongolia cashmere goat, Liaoning cashmere goat and Wendeng diary goat . The 4 pairs of primers for FGF5 gene were designed based on the human and mouse genomic sequence. Two SNPs were identified in the goat FGF5 gene by PCR-SSCP and sequencing. They were in the exon 1 region in different goat lines. In primer 3 and primer 4 no SNP. The sequence data showed that two SNPs found in the exonl region of the gene gene were A to T and C to T. Population genetic analysis indicated that genotype frequency of the P1 SNPs was similar in different breed, gene B is mean gene. All the population were in Hardy-Weinberg equilibrium state . For primer 2, frequency of the EE genotype in both cashmere goat was higer than in the Wendeng lines, Frequency of allele D in primer 2 in Wendeng goat was higher than that of other breeds. Both cashmere population were in Hardy-Weinberg equilibrium state but Wendeng goat was not in Hardy-Weinberg equilibrium state.
出处
《华北农学报》
CSCD
北大核心
2006年第3期71-76,共6页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学地区重点基金
中国农业大学农业生物技术国家重点实验室联合资助(39969002)
