摘要
建立了一种毛细管电泳测定左旋多巴药片光学纯度的新方法.D,L-多巴（D,L-dopa）用2,4-二硝基氯苯（CDNB） 衍生,以β-环糊精（β-CD）为手性选择剂,在硼砂（pH 9.0）缓冲溶液中进行毛细管电泳手性分离.采用紫外检测,测定波长为220 nm.在测定条件下,对映体分离在9 min内完成.L-dopa中D-dopa的含量在0.10%～2.0%范围内,与D-dopa的峰面积呈现良好的线性关系.在L-dopa中加入不同量的D-dopa,测得回收率在96%～102% 之间,每个样品分别测定3次,其相对标准偏差（RSD）小于2.3%.方法用于市售左旋多巴药片的分析,测得左旋多巴药片中D-dopa的含量为0.20%.
A new capillary electrophoresis method was established for the optical purity analysis of levodopa. D,L-dopa was derivatized with 1-chloro-2,4-dinitrobenze （CDNB） and separated by capillary electrophoresis using β- cyclodextrin （β-CD） as chiral selector in a borate buffer at pH 9.0, followed by direct ultraviolet absorptive measurement at 220 nm. Under optimal conditions, enantiomeric separation of D, L-dopa was completed within less than 9 min, and the linear relationship between the peak area of D-dopa and the content of D-dopa in L-dopa was found in the range of 0. 10% -2.0%. The recovery for standard addition was estimated within the 96.0% - 102.0%. The relative standard deviation, for three independent analyses of each sample, was less than 2.3%. The presented method has been applied to the analysis of real levodopa drug, and allowed the determination of 0.20% D-dopa in levodopa with well peak identification.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第6期859-862,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(No.20265001)
广西高校基金项目资助
关键词
左旋多巴
毛细管电泳
对映体分离
光学纯度检验
Levodopa, capillary electrophoresis, enantiomeric separation, optical purity analysis
