摘要
为了降低木质纤维素水解液发酵抑制剂对乙醇发酵的负影响,采用混合菌种对木质纤维素稀酸水解液乙醇发酵方式进行了研究。对批式发酵、补料批式发酵和间隔补料批式发酵3种发酵方式进行了比较。实验结果表明,间隔补料批式发酵可以有效地减弱水解液中抑制因子对菌种的影响,乙醇产量明显高于其他两种发酵方式,利用酿酒酵母(Saccaromyces cerevisiae 2.535)和嗜鞣管囊酵母(Pachysolen tannophilis ATCC 32728)混合发酵,乙醇产量最终达到14.4g/L,乙醇产率(Yp/s)为0.47g/g,相当于最大理论产率的92.2%。利用酿酒酵母和重组大肠杆菌混合菌种发酵,乙醇产量达到了14.5g/L。对木质纤维素稀酸水解液采用间隔补料批式乙醇发酵方法,可进一步减少抑制剂对乙醇发酵的影响,使发酵顺利进行。
In order to reduce negative effect of inhibitory compounds in bioconversion process of diluted-acid hydrolysis of lignocellulose to ethanol the fermentations were carried out with cocultures and batch, fed-batch and intermittent fedbatch fermentation methods. By the intermittent fed-batch fermentation, fermenting with coculture Saccaromyces cerevisiae 2.535 and Pachysolen tannophilis ATCC 32728, the ethanol concentration was 14.4g/L, and the ethanol yield（Yp/s） was 0.47g ethanol/g sugar, corresponding to 92.2% of theoretical ethanol yield. Fermenting with coculture Saccaromyces cerevisiae 2.535 and recombinant Escherichia coli, the ethanol concentration was 14.5g/L. The demonstration indicates that the new method results in higher ethanol yield than that of other two methods and reduces the impact inhibitory concentration effectively.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2006年第6期618-622,共5页
Acta Energiae Solaris Sinica
基金
国家高科技发展计划(863)(2001AA514024和2002AA514010)
北京市教委项目
关键词
木质纤维素乙醇发酵
混合菌种
补料批式发酵
间隔补料批式发酵
diluted-acid lignocellulosic hydrolysate ethanol fermentation
coculture
fed-batch fermentation
intermittent fed-batch fermentation
