摘要
采用去卵巢(OVX)SD大鼠模拟女性绝经后神经元的退变来观察雌激素、植物雌激素对海马神经元线粒体的保护作用。雌性SD大鼠分为5组:(1)正常对照组;(2)去卵巢对照组;(3)雌激素治疗组;(4)金雀异黄酮治疗组;(5)依普拉芬治疗组,并于术后12d取材进行超微结构观察和体视学定量分析。结果显示:(1)去卵巢后海马神经元的线粒体肿胀明显,嵴断裂明显,空泡化显著;3个治疗组细胞和线粒体相对于去卵巢对照组均有明显改善;(2)与去卵巢对照组相比较,3个治疗组线粒体的体积密度(Vv)、平均直径(D)、平均表面积(S)明显减小(P<0.05);3个治疗组的比表面(δ)、数密度(Nv)和粒子分散度(Cλz)较去卵巢对照组明显增大(P<0.05)。以上结果表明:雌激素和植物雌激素对去卵巢后大鼠的海马神经元线粒体具有保护作用。本研究结果为雌激素和植物雌激素以线粒体为靶点防治女性更年期后神经元的退变及老年性痴呆可能的作用机制提供了一定的形态学资料。
In this study, the ovariectomized （OVX） animal model of Sprague - Dawley rats were used to imitate the pathologic changes of neural degeneration of postmenopausal women. The estrogen and phytoestrogen were given to model rats for observing the protective effect to the mitochondrla of hippocampal neurons. The rats were randomly divided into 5 groups： （ 1 ） normal control group; （2） OVX 12-day （OVX-12） model group; （3） estrogen treatment group; （4） genistein treatment group; （5） iprinavone treatment group. After 12 days, the uhrastructure change of mitochondria of hippocampal neurons of rats were observed and made a stereological analysis. The results showed ： （ 1 ） the mitochondria swelled obviously, like empty-bubble, and the cristas broke and reduced or disappeared largely in the OVX-12 group, but the mitochondria were protected well, and the changes were like those of normal control group in the estrogen and phytoestrogen treatment group. （2） The volume density （Vv） , average diameter （D） and average surface area （S） of mitochondria declined obviously （P〈0.05 ） , and surface area ratio, number density （ Nv）, particle dispersity （Cλz） of the three treatment groups aggrandized （P〈0.05） comparing with the OVX-12 group. These results suggest that estrogen and phytoestrogen could withstand the degeneration of mitochondria better, which hinted that the mechanism of estrogen and phytoestrogen on the neural degeneration and Alzbeimer＇s disease after menopause could be explained via protecting the functions of mitochondria.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2006年第3期351-355,共5页
Chinese Journal of Neuroanatomy
基金
广东省中医药局科研项目(No.A402013)
广东省自然科学(No.C04105332)
广东省科技计划重点引导项目(No.2005B33801004)资助项目
关键词
植物雌激素
去卵巢
超微结构
线粒体
体视学
phytoestrogen, ovariectomized, uhrastructure, mitochondria, stereology
