摘要
目的:探讨热敏环境培养变形链球菌后,与唾液分泌性免疫球蛋白A(SIgA)的免疫反应性。方法:从20例志愿者口腔中收集牙菌斑,分离临床变形链球菌,生化实验和PCR鉴定临床变形链球菌的血清型,随机引物多聚酶联反应(AP-PCR)鉴定基因型。每种基因型菌株分2组培养:实验组42℃厌氧培养;对照组37℃厌氧培养。用改良唾液收集器无菌收集非刺激性下颌下腺、舌下腺唾液,免疫印迹分析每位志愿者的唾液SIgA与自身临床菌株和参考菌株的免疫反应性。结果:20例成人共检出21种不同基因型的C型变形链球菌。实验组与对照组的变形链球菌与唾液SIgA的免疫印迹无显著差异,仅个别杂交带的密度强弱有改变。同一个体的唾液SIgA对不同基因型的菌株,出现不同的免疫印迹带;同一参考菌株与不同个体的唾液SIgA有不同的免疫印迹。结论:人群口腔变形链球菌存在多种基因型,具有遗传多样性;变形链球菌存在多种抗原成分,可以与唾液SIgA发生免疫反应,并具有个体差异;虽然变形链球菌在热敏环境中有热休克蛋白的产生,但是与唾液SIgA没有反应原性或免疫反应性较弱。
PURPOSE： The purpose of this study was to compare the salivary immunoglobulin A antibody response to Streptococcus mutans in normal with in heat treated stress. METHODS： Clinical Streptococcus mutans strains were isolated from 20 volunteers, serotyped by biochemical test and PCR, and genotyped by AP-PCR. Unstimulated secretions from submandibular glands and sublingual glands were collected from volunteers by modified collectors. Each identified genotype was cultured in two groups： control group was grown in BHI broth at 37℃ for 3 hours; stress group was incubated in BHI broth at 42℃ for 3 hours. Analysis of SIgA activity to clinical genotype strains and reference strains in different group was detected by Western blot. RESULTS： There was no significant difference between stress group and control group,in spite that some bands had strong or weak intensity.Different genotypes of S.mutans could have different immunoblotting profile as for an individual. SIgA from different volunteers could have different immonoblotting profiles as to the same genotype strain. CONCLUSIONS： Although Streptococcus mutans can express heat shock proteins in stress, this study suggests these new proteins have no significant effect on the reaction of SIgA to Streptococcus mutans. Different genotype strains may have different proteins, and different immunoreactivity to host. Different hosts may have different immunoreactivities to one genotypes of S.mutans. Supported by National Tenth Five-Year Key Project （Grant No.2004BA720A24）; Creative Team Plan of Natural Science Foundation of Hubei Province （Grant No. 2004ABC004） and Chenguang Plan of Wuhan City （Grant No. 20035002016-21）.
出处
《上海口腔医学》
CAS
CSCD
2006年第3期285-289,共5页
Shanghai Journal of Stomatology
基金
"十五"国家科技攻关计划项目(2004BA720A24)
湖北省自然科学基金创新群体项目(2004ABC004)
武汉市晨光计划项目(20035002016-21)
