摘要
目的 建立高效液相色谱（HPLC）法测定血清头孢唑肟浓度。方法色谱柱为μbondaparC18（7．8mm×300mm，5μm），流动相：甲醇-0.01mol·L^-1磷酸二氢钾（20：80），内标为丙酮，检测波长：254nm，柱温：室温，流速：1．0mL·min^-1。结果头孢唑肟的血药浓度在10-20μg·mL^-1范围内与峰面积比有良好的线性关系。血清中头孢唑肟在24h内约降解60．0％。结论 该方法简单，快速，准确，为头孢唑肟的血清浓度监测提供了分析方法。
Objective To set up high performancing liquid chromatography（HPLC） method for determining the serum concentration of ceftizoxime. Methods The chromatographic colu.mn was a μbondapar C18 （7.8 mm × 300 mm, 5μm）, the mobile phase consisted of methanol-0.01mol·L^-1 potassium dihydrogen phosphate （20 ： 80）, the detecting wave-length was 254nm, the flow rate was 1.0 mL·min^-1, and the column was kept at the room temperature. Results The determination limit was 0. 1μg·mL^-1. The linear relationship was fairly good with the amount of ceftizoxime within the range of 10 - 20μg·mL^-1 （ r = 0. 999 5 ）. The average rate of recovery was no more than 6.8%. Conclusion The HPLC method is simple, rapid, and accurate for determining the serum concentration of ceftizoxime.
出处
《医药导报》
CAS
2006年第7期632-633,共2页
Herald of Medicine
