摘要
酪氨酸酶(TYR)、酪氨酸酶相关蛋白1(TYRP1)和酪氨酸酶相关蛋白2(TYRP2)是动物黑色素生物合成过程的重要酶,黑素皮质素1受体(MC1R)一旦与配体α-促黑素细胞激素(α-MSH)结合,对TYR、TYRP1和TYRP2酶活性的表达水平均有重要影响,因此认为MC1R是黑素合成模式的控制点。本研究以MC1R基因为候选基因,对乌骨绵羊MC1R基因多态性进行了研究,希望揭示MC1R基因与乌骨绵羊乌质性状之间的关系。结果表明,乌骨绵羊MC1R基因存在2个多态位点,分别为A12G和G144C。翻译表明这2处突变都为同义突变,对应的氨基酸分别是丝氨酸(Ser)和亮氨酸(Leu)。PCR-RFLP分析发现绵羊MC1R存在2个等位基因,即MC1R*1和MC1R*2,对应11型、22型和12型3种基因型。乌骨绵羊和兰坪本地绵羊MC1R等位基因的频率差异不显著,而与罗姆尼羊差异显著,推测MC1R基因可能不是控制乌骨绵羊乌质性状的主效基因。
TYR, TYRP1 and TYRP2 are key enzymes in melanin biosynthesis. Once combining its receptor, MC1R should affect enzyme activity of TYR, TYRP1 and TYRP2. So MC1R is a key control of melanin biosynthesis. We selected the MC1R gene as a candidated gene for melanin trait of sheep and studied MC1R gene polymorphism. The results showed that there were two nucleotide mutation sites of MC1R gene of black bone sheep, A12G and G144C, respectively. But they were both synonymous mutation and their corresponding amino acids were Ser and Leu. We found two alleles by PCR-RFLP （MC1R＊1 and MC1R＊2） , corresponding three genotype, 11, 22 and 12, respectively. There was no significant difference of MC1R allele frequency between black bone sheep and native sheep in Lanping （ P 〉 0. 05 ） , while the frequency in black bone sheep was significant higher than that in Luomuni sheep （P 〈0. 05）. It indicated that MC1R gene was not a dominating effective gene which controlled melanin trait of black bone sheep.
出处
《畜牧与兽医》
北大核心
2006年第4期5-7,共3页
Animal Husbandry & Veterinary Medicine
基金
国家自然基金面上项目(30460089)
云南省自然科学基金资助项目(2002C0038M)
