摘要
目的研究核因子-κB(NF-κB)在糖尿病(DM)大鼠坐骨神经中的表达及还原型谷胱甘肽(GSH)的干预作用。方法将36只大鼠随机分为对照组(NC组)、糖尿病组(DM组)和糖尿病还原型谷胱甘肽处理组(DG组)。后两组以链脲佐菌素(STZ)法制备DM大鼠动物模型,DG组同时以GSH腹腔内注射,12周后以电泳迁移率变动分析技术(EMSA)检测坐骨神经组织中NF-κB活性表达的变化。结果DM组大鼠坐骨神经组织中NF-κB的表达较对照组明显增加(P<0·05);以GSH处理12周后,NF-κB活性降低(P<0·05),坐骨神经组织病理学改变改善。结论实验性DM大鼠坐骨神经组织中NF-κB活性增加,而GSH在一定程度上抑制其活化,可能对预防糖尿病神经病变的发生有一定作用。
Objective To investigate the expression of NF-κB and the effect of GSH on NF-κB in sciatic nerve of diabetic rats. Methods Thirty-six rats were randomly divided into control group （NC）, diabetic group （DM） and GSH treatment group （DG）. The rats of DM group and DG group were intraperitoneally injected streptozotozin （STZ）. At the end of the 12 th week, the sciatic nerves were taken out from all rats for measuring the NF-κB activity by electrophoretic mobility shift assays （ EMSA ）. Results The expression of NF-κB was markedly increased in DM group than that in NC group. After treated with GSH, the expression of NF-κB was increased and the sciatic nerve injury improved. Conclusion The expression of NF-κB is increased in diabetic rats. GSH may partly suppress the expression of NF-κB in the sciatic nerve of diabetic rats.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第7期645-647,共3页
Jiangsu Medical Journal
