摘要
目的:目的建立辽宁嗜人按蚊和广东嗜人按蚊实验室品系,观察其生物学特性。方法:采用人工交配繁殖,控温、控湿,加强自然光照,幼虫饲以颗粒小鱼饲料,成蚊以豚鼠饲血。对两地嗜人按蚊生长发育不同时期进行逐日记录观察。结果:控温、控湿在28℃±2℃、75%~85%,以及充足光照的情况下,两种嗜人按蚊平均孵化率、幼虫成活率、蛹化率、羽化率,辽宁嗜人按蚊为74.10%、84.44%、91.26%、92.70%;广东嗜人按蚊为79.93%、77.88%、92.87%、96.07%。现已养育繁殖37个世代。结论:辽宁嗜人按蚊和广东嗜人按蚊实验室种群建立后,生物学特性稳定,可用于嗜人按蚊的多种实验研究。
objective: To establish Liaoning and Guangdong laboratory populations of An. Anthropophagus and to observe their biological characteristics. Methods: Artificial copulation was used for raproduction . The room temperature , air humidity were controlled and natural illumination strengthened during laboratory breeding . The mosquito larval were fed with particulate fry fooler and adults with blood of guinea pig. The developing process in laboratory was recorded day by day . Results: Under the room temperature of 28℃2 ±2℃ , humidity of 75% -85%, and full of natural illumination , the rats of average hatching , larva survival , pupation , and eclosion were 74.10%, 84.44%, 91.26%and 92.70%of Liaoning population, 79.93%, 77.88 %, 92.87%and 96.07 % of Guangdong population. Up to now 37 generation have been bred. Conclusion: The Liaoning and Guangdong laboratory populations of An. Anthropophagus with stable biological characteristics have successfully been established in our laboratory .
出处
《现代预防医学》
CAS
北大核心
2006年第7期1305-1307,共3页
Modern Preventive Medicine
基金
国家"十五"科技攻关资助项目(2001BA705B09)
关键词
嗜人按蚊
实验室种群
生物学特性
Anopheles Anthropophagus
Laboratory population
Biological characteristic