摘要
目的：了解临床分离的80株大肠埃希菌耐药性及氨基糖苷类修饰酶（AMEs）基因的存在状况。方法：采用MH平板法测试14种抗菌药物的敏感性，采用PCR技术检测氨基糖苷类修饰酶基因。结果：80株大肠埃希菌呈现多重耐药，对氨基糖苷类抗生素的耐药率在57．5％-80％之间，氨基糖苷类修饰酶基因aac（3）-I、aac（3）-Ⅱ、aac（6’）-I、ant（3”）-I基因的阳性率分别为20％、30％、32．5％、20％。携带1种或1种以上基因的菌株有65株（81．25％）。结论：临床分离的大肠埃希菌多重耐药严重，氨基糖苷类修饰酶基因携带率较高。
Objective： To investigate the resistance and genotyping of aminoglycoside modifying enzymes （AMEs） in Escherichia coli isolated from the clinic. Methods： M-H Tests were performed to detect the susceptibility of 14 kinds of antimicrobial agents against 80 strains of Escherichia coli. Genotypes of AMEs was analyzed by polymerase chain reaction（PCR）. Results： Eighty strains were multiple-drug-resistance. The resistant rates to aminoglycosides ranged from 57.5% to 80% ,AMEs gene was found in 42.5%. The positive rates of AMEs genes of aac（3）- I ,aac（3）- Ⅱ ,aac（6＇）- I ,ant（3＂）- I were 20%, 30% ,32.5% and 20%, one of genes or some of genes were 65 strains （81.25%＇）. Conclusion： The muhidrug resistance Escherichia coli isolated from the clinic was a serious issue, and there were higher positive percentages genotyping of aminoglycoside modifying enzymes （AMEs） in Escherichia coli.
出处
《江苏大学学报（医学版）》
CAS
2006年第3期246-247,250,共3页
Journal of Jiangsu University：Medicine Edition
关键词
大肠埃希菌
氨基糖苷类修饰酶
基因
Escherichia coli
aminolycoside modifying enzymes
gene
