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荧光实时定量PCR的研究进展 被引量：14

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作者 张驰宇 成婧 李全双 曹威 孙金燕
机构地区 江苏大学医学技术学院
出处 《江苏大学学报（医学版）》 CAS 2006年第3期268-271,共4页 Journal of Jiangsu University：Medicine Edition
基金 江苏大学第四批大学生科研资助项目(A095) 江苏大学高级人才科研启动项目(2281270002)
关键词 荧光实时定量PCR 相对定量 绝对定量 扩增效率
分类号 R446.61 [医药卫生—诊断学]
  • 相关文献

参考文献14

  • 1Giulietti A,Overbergh L,Valckx D,et al.An overview of real-time quantitative PCR:applications to quantify cytokine gene expression[J].Methods,2001,25 (4):386 -401.
  • 2Schmittgen TD,Zakrajsek BA,Mills AG,et al.Quantitative reverse transcription-polymerase chain reaction to study mRNA decay:comparison of endpoint and realtime methods[J].Anal Biochem,2000,285 (2):194 -204.
  • 3Klein D.Quantification using real-time PCR technology:applications and limitations[J].Trends Mol Med,2002,8(6):257 -260.
  • 4Mackay IM,Arden KE,Nitsche A.Real-time PCR in virology[J].Nucleic Acids Res,2002,30 (6):1292 -1305.
  • 5Bustin SA.Absolute quantification of mRNA using realtime reverse transcription polymerase chain reaction assays[J].J Mol Endocrinol,2000,25 (2):169-193.
  • 6Bustin SA.Quantification of mRNA using real-time reverse transcription PCR (RT-PCR):trends and problems[J].J Mol Endocrinol,2002,29(1):23-39.
  • 7Tyagi S,Kramer FR.Molecular beacons:probes that fluoresce upon hybridization[J].Nat Biotechnol,1996,14(3):303-308.
  • 8Whitcombe D,Theaker J,Guy SP,et al.Detection of PCR products using self-probing amplicons and fluorescence[J].Nat Biotechnol,1999,17 (8):804-807.
  • 9Wittwer CT,Herrmann MG,Moss AA,et al.Continuous fluorescence monitoring of rapid cycle DNA amplification[J].Biotechniques,1997,22(1):130-131,134-138.
  • 10张驰宇,张高红,杨敏,贲昆龙.四步法消除SYBR GreenⅠ实时定量RT-PCR中引物二聚体的影响[J].中国生物化学与分子生物学报,2004,20(3):387-392. 被引量：47

二级参考文献15

  • 1徐顺高,黄新祥,周丽萍.荧光实时定量RT-PCR观察伤寒杆菌鞭毛z66和d/j抗原基因表达方法的建立[J].江苏大学学报（医学版）,2005,15(1):21-24. 被引量：3
  • 2Bustin S A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol,2000,25(2): 169 - 193
  • 3Giulietti A, Overbergh L, Valckx D, Decallonne B, Bouillon R,Mathieu C. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression. Methods, 2001, 25(4): 386 - 401
  • 4Mackay I M, Arden K E, Nitsche A. Real-time PCR in virology.Nucleic Acids Res, 2002, 30(6): 1292 - 1305
  • 5Brownie J, Shawcross S, Theaker J, Whitcombe D, Ferrie R, Newton C, Little S. The elimination of primer-dimer accumulation in PCR.Nucleic Acids Res, 1997, 25(16): 3235 - 3241
  • 6Halford W P, Falco V C, Gebhardt B M, Carr D J. The inherent quantitative capacity of the reverse transcription-polymerase chain reaction. Anal Biochem, 1999, 266(2): 181 - 191
  • 7Vandesompele J, De Paepe A, Speleman F. Elimination of primerdimer artifacts and genomic coamplification using a two-step SYBR green I real-time RT-PCR. Anal Biochem, 2002, 303( 1 ): 95 - 98
  • 8Ririe K M, Rasmussen R P, Wittwer C T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction.Anal Biochem, 1997, 245(2): 154 - 160
  • 9Morrison T B, Weis J J, Wittwer C T. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification.Biotechniques, 1998, 24(6): 954 - 962
  • 10Bustin S A. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol, 2000, 25 (2): 169～193.

共引文献171

同被引文献181

引证文献14

二级引证文献115

江苏大学学报（医学版）
2006年 第3期

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