摘要
将低密度脂蛋白和脂蛋白（ａ）用神经氨酸酶处理，去除低密度脂蛋白（ａ）中所含末端唾液酸，然后用１２５Ⅰ－纤维素二糖酪胺标记，经刺猬腋下静脉注射，分别于３ｈ和６ｈ处死，测定血、肝、脾、肾和胆汁中放射含量，分析和比较去唾液酸和富含唾液酸低密度脂蛋白和脂蛋白（ａ）的代谢变化。实验发现：注射去唾液酸低密度脂蛋白的最初３ｈ，组织内的代谢速率低于低密度脂蛋白组，而６ｈ后代谢速率则高于低密度脂蛋白组。注射去唾液酸蛋白（ａ）３ｈ和６ｈ在组织中代谢速率均显著高于脂蛋白（３）组。低密度脂蛋白和去唾液酸低密度脂蛋白均能被脾脏中巨噬细胞吞噬后蓄积在细胞内，脂蛋白（ａ）虽然能被脾脏巨噬细胞吞噬，但去唾液酸脂蛋白（ａ）很少进入脾细胞，既使进入也很快被清除。
Aim We used hedgehogs to study the effects of sialic acid in the structure of low density lipoprotein (LDL)and lipoprotein (a)[Lp(a)] on the metabolism of the LDL and Lp(a) with 125 iodine. Methods The end sialic acid on the stucture of LDL and lipoprotein (a) were removd with the treatment of neuraminidase.The desialylated lipoprotein (a) (ds-Lp (a), desialylated low density lipoprotein (ds-LDL),LDL and Lp (a)were marked with 125 iodine tyramine cellobiose. They were injected into the body of the hedge hogs via the armpil vein. The animals were put to death in 3 h and 6 h.The concentration of radioactivity in the blood, liver, spleen,kidney and gall were measured. The change of metabolism of ds-LDL and ds-Lp (a) were analyzed and compared with that of norma LDL and Lp (a). Results The experiments showed that the rate of the metabolism of ds-LDL in 3 h is 7.6% lower than that of LDL,and the rate of the metabolism of ds-LDL in 6 h is 12.23% higher than that of LDL. On the contrary the concentrations of ds-Lp (a) in all the organs reduced by 2 times and 3.85 times to the concentrations of Lp (a)in 3h and 6h respectively,LDL and ds-LDL canbe swallowed by the spleen cells and stored. Although Lp (a) can alsO be swallowed by the spleen cells,ds-LP (a) had a little to enter the spleen cells. Even so it can be removed quickly.Conclusions Experiments proved that sialic acid plays a very important role in the metabolism of Lp (a)and remaining stable of the structure of Lp (a).
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第2期115-118,共4页
Chinese Journal of Arteriosclerosis
关键词
低密度脂蛋白
脂蛋白
去唾液酸
动脉粥样硬化
Desialylated lipoprotein (a)
Desialylated low density lipoprotein
Lipoprotein (a)
Low density lipoprotein
Hedgehog
