摘要
目的探讨充血性心力衰竭(CHF)患者外周血单个核细胞(PBMCs)核因子-κΒ(NF-κΒ)表达与肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)分泌的相关性。方法25例心力衰竭患者和15例健康对照组PBMCs NF-κΒ表达由免疫组化染色检测,血清细胞因子的含量由放射免疫法测定。对两组的指标进行t检验及相关分析。结果心力衰竭患者PBMCs NF-κΒ胞核染色阳性率和血清细胞因子(TNF-αI、L-1βI、L-6)含量显著高于健康对照组(P<0.01),且NFκ-Β胞核染色阳性率和血清细胞因子(TNF-αI、L-1βI、L-6)含量呈显著正相关(P<0.05)。结论充血性心力衰竭患者NFκ-Β表达增高,细胞因子(TNF-αI、L-1βI、L-6)分泌增多。在充血性心力衰竭,可能通过NF-κΒ表达的上调促进细胞因子(TNF-αI、L-1βI、L-6)的分泌。
Objective To study the relationship of NF-kB activation on peripheral blood mononuclear cells （PBMCsJand the levels of TNF-α.IL-1β.IL-6 in heart failure patients. Methods twenty-five heart failure patients and fifteen age and gender matched healthy subject were included in the study. TNF-α.IL-1β and IL-6 concentrations were measured using radioimmuity assay .The activity of NF-kB were measured by immunocytochemical staining. Results Compared with the healthy subject,NF-kB activation,TNF-α.IL-1β and IL-6 levels in heart failure patients were found to be increased（ P 〈 0.01 ）. NF-kB activation were positively correlated with the levels of TNF-α.IL-1β and IL-6（ P 〈 0.05）. Conclusion NF-kB activation, the levels of TNF-α. IL-1β and IL-6 were increased in heart failure patients. NF-kB upregulates the the levels of TNF-α.IL-1β and IL-6 in heart failure patients.
出处
《四川医学》
CAS
2006年第7期706-707,共2页
Sichuan Medical Journal
