摘要
目的建立一种适宜基层地区的快速分析多种喹诺酮类药物的HPLC法。方法色谱柱AlltechAlltimaC18(150mm×4.6mm,5μm);柱温为室温;流动相:柠檬酸(0.2mol/L)∶(甲醇∶乙腈=3∶1)(84∶16);流速1.0ml/min;检测波长275nm;进样量10μl。结果帕珠沙星、洛美沙星、左氧氟沙星的线性范围分别为0.08～0.40mg/ml、0.04～0.20mg/ml和0.04～0.20mg/ml;平均回收率分别为99.3%、98.6%、101.1%;最低检测限分别为33.0、8.2和9.6ng。结论本方法快速简便,准确度可靠,可作为快速检验帕珠沙星、洛美沙星、左氧氟沙星制剂含量的方法。
Objective To establish a method for fast simultaneous determination of pazufloxacin, lomefloxacin and levofloxacin by HPLC for the basic unit laboratories. Method The analytical column was Alltech Alhima C18（150mm×4.6mm,5μm）; column temperature： room temperature; the mobile phase was a mixture of 0. 2mol/L citric acid： （methanol： acetonitrile = 3： 1） = 84： 16; the flow rate was 1.0ml/min, the detection wavelength was 275nm and the injection volume was 10p.1. Results The calibration curve for pazufloxacin, lomefloxacin and levofloxacin were linear in the range 0. 08 - 0. 40mg/ml, 0. 04 - 0. 20mg/ml and 0. 04 - 0. 20mg/ml, respectively. And the average recovery rate were 99.3% ,98.6% and 101.1%; the detection limit were 33ng,8.2ng and 9.6ng, respectively. Conclusion The method was convenient, specific and accurate for the fast simultaneous determination of those drugs in the basic unit laboratories.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2006年第7期420-422,共3页
Chinese Journal of Antibiotics
