摘要
以同源四倍体核不育水稻为研究材料,以氮离子束为诱变源完成了离子注入试验。对离子注入群体进行筛选后以变异单株为基础,在育性分化的敏感期经过低温处理后对其育性表达特性进行了研究。研究结果表明,在氮离子注入剂量为1×1017cm-2的处理中,在同源四倍体核不育水稻的当代群体内出现了比较多的变异单株。在各个变异单株之间其育性表达发生了明显的变异。在育性分化的敏感期经过低温处理后,某些突变体在伴随着发生形态学变异的同时也会发生相应的育性变异,而另一些突变体在发生形态学变异或生育特性变异的情况下仍然保持着其亲本的雄性不育特性。在M3代群体内,各个株系群体内单株之间的农艺性状并没有达到整齐一致的状态,但各个株系在雄性不育性表达上却保持了上一个世代的突变特性。由此认为,氮离子束注入所导致的育性变异在M3代群体内并没有发生进一步的分离。
The N^＋ implantation experiment, taking autotetraploid rice with nucleus male sterility as materials, was finished. The off-spring colonies via N^＋ implantation were selected, gaining some mutants in autotetraploid rice. The characters of infertility expression of the variant single plants, treated by the abnormal low temperature during male differentiation, were studied. As a result of 25 keV N^＋ion implantation with a low dose （1 × 10^17 cm^2）, there were some variant single plants in the autotetraploid rice. The variant single plants represented specially in infertility. Treated by the abnormal low temperature during male differentiation, some mutants showed obviously variant characters in infertility following the morphological variation, but others preserved infertility of its parent. The segregation phenomena in the agronomic characters were visible in the lines of M3 generation, but they kept the stable infertility. It is thought that the characters of the infertility variation in the mutants may be allocated in the M3 generation.
出处
《核技术》
EI
CAS
CSCD
北大核心
2006年第7期513-517,共5页
Nuclear Techniques
基金
国家"十五"科技攻关项目(2001BA302B)
关键词
同源四倍体水稻
核雄性不育
氮离子注入
育性表达
Autotetraploid rice, Nucleus male sterility, N^＋ion implantation, Infertility expression
