摘要
目的建立BCL11B基因的定量检测方法,并分析其在白血病中的表达水平。方法利用实时定量RTPCR分析TALL(12例)、BALL(8例)、BCLL(6例)、AML(7例)和正常对照(10例)外周血单个核细胞(PBMC)中BCL11B基因的表达水平,以β2微球蛋白(β2M)基因表达水平作为内对照。结果TALL患者PBMC中BCL11B表达水平(553.84±564.01拷贝105β2M拷贝)明显高于正常对照和其他白血病患者(P=0.006,P=0.013,P=0.031,P=0.020);而AML组BCL11B表达水平(0.02±0.04拷贝105β2M拷贝)则明显低于正常对照组(P=0.000)、BALL组(P=0.006)和TALL组(P=0.020);BALL(1.99±1.59拷贝105β2M拷贝)和BCLL(2.26±3.57拷贝105β2M拷贝)中BCL11B表达水平与正常对照(2.20±1.01拷贝105β2M拷贝)无显著差别。结论建立了实时定量RTPCR检测BCL11B方法,TALL中BCL11B高表达可能与其发病有一定关系。
Objective To establish the quantitative detection method for BCLllB gene which is a key regulator of both differentiation and survival during T cell development, and to evaluate its expression level in leukemia. Methods Real-time RT-PCR was performed to detect the expression level of BCLllB gene in peripheral blood mononuclear cells from 10 normal individuals （as control） and patients with T-ALL （12 cases）, B-ALL （8 cases）, B-CLL （6 cases）, and AML （7 cases）. The expression level of β2 microglobuline gene （β2 M） was used as referenee to confum the expression level of BCLllB. Results The expression level of BCLllB in T-ALL （553.84±564.01 copies/10^5 β2M copies） was significant higher than those in the normal control （2.20 ±1.01 copies/10^5β2M copies） and the other leukemia groups （ P = 0.006, P = 0.013, P = 0.031, P = 0.020, respectively）, whereas the expression level of BCLllB in AML （0.02 ± 0.04 copies/los β2M copies） was significantly lower than that in the other groups （ P = 0.000, P = 0.006, P = 0.020, respectively）. The expression levels of BCL11B in B-ALL （1.99 ± 1.59 copies/los β2M copies） and B-CLL （2.26 ±3.57copies/los β2M copies） had no significant difference compared with the control group. Conclusion The detection method for BCL11B gene expression by real-time RT-PCR analysis is established. The overexpression of BCLllB gene in T-ALL may relate to its pathogenesis.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第4期463-465,共3页
Immunological Journal
